STOCK Roratm1(cre)Ddmo/Cnrm
| Status | Available to order |
| EMMA ID | EM:13253 |
| International strain name | STOCK Roratm1(cre)Ddmo/Cnrm |
| Alternative name | RORalpha-IRES-Cre |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Roratm1(cre)Ddmo, |
| Gene/Transgene symbol | Rora |
Information from provider
| Provider | Silvia Kirsten Nicolis |
| Provider affiliation | Biotechnology and Biosciences, University of Milano-Bicocca |
| Additional owner | Dr. Shen-Ju Chou, Academia Sinica Taiwan. Dr. Michèle Studer, University of Nice-Cote d'Azur, France. Dr. Dennis O'Leary, Professor Emeritus, Salk Institute, US. |
| Genetic information | The RORalpha-IRES-Cre transgene was generated by insertion of an IRES/Cre/FRT/neo/FRT cassette at the XbaI site 20bp downstream of the stop codon in the 3'UTR of the RORalpha gene; the resulting targeting vector was used to produce an IRES Cre "knock-in" downstream to the resident RORalpha gene by gene targeting (Chou S-J et al, Science 2013, PMID: 23744949). RORalpha-IRES-Cre is active in the neurons of the sensory nuclei of the developing thalamus, including the visual thalamic nucleus (dorsolateral geniculate nucleus, dLGN) from embryonic day (E) 14.5 (Chou et al, PMID: 23744949; Mercurio S et al, iScience 2019, PMID: 31082736). |
| Phenotypic information | Homozygous:The mutation consists in the targeted insertion of IRES-Cre downstream to the RORalpha gene, whose sequence is untouched by the mutation. Therefore it is expected that homozygotes are normal.Heterozygous:No detected phenotype |
| Breeding history | The transgene was received from Dr. Michèle Studer (Nice, France), who had bred it with a Nr2f1flox mutation to generate a Nr2f1 (also called COUP-TF1) conditional knockout, as described in (Chou S-J et al, Science 2013, PMID: 23744949). We bred the transgene to a Sox2flox mutation (Favaro et al, Net Neurosci 2009, PMID: 19734891) to obtain a Sox2 conditional knockout, described in Mercurio S et al, iScience 2019, PMID: 31082736). We maintained the line from that time by breeding of compound RoralphaCre-Sox2flox carriers (viable and fertile); the RORalpha-IRES-Cre allele was initially derived in R1 ES cells (129-derived); the Sox2flox mutation was derived in CJ7 ES cells (129-derived); chimaeras had been bred to C57BL/6J or B6D2F1 females to obtain carrier offspring. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Geniculocortical input drives genetic distinctions between primary and higher-order visual areas.;Chou Shen-Ju, Babot Zoila, Leingärtner Axel, Studer Michele, Nakagawa Yasushi, O'Leary Dennis D M, ;2013;Science (New York, N.Y.);340;1239-42; 23744949
- Sox2 Acts in Thalamic Neurons to Control the Development of Retina-Thalamus-Cortex Connectivity.;Mercurio Sara, Serra Linda, Motta Alessia, Gesuita Lorenzo, Sanchez-Arrones Luisa, Inverardi Francesca, Foglio Benedetta, Barone Cristiana, Kaimakis Polynikis, Martynoga Ben, Ottolenghi Sergio, Studer Michèle, Guillemot Francois, Frassoni Carolina, Bovolenta Paola, Nicolis Silvia K, ;2019;iScience;15;257-273; 31082736