B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes Tg(tetO-Cpt1a,-EGFP)/Cnbc

Status

Available to order

EMMA IDEM:13348
International strain nameB6.Cg-Tg(Pax8-rtTA2S*M2)1Koes Tg(tetO-Cpt1a,-EGFP)/Cnbc
Alternative namePax8-rtTAtg/0:tetO-Cpt1atg/0 (Pax8-CPT1A)
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Pax8-rtTA2S*M2)1Koes,
Gene/Transgene symbolTg(Pax8-rtTA2S*M2)1Koes

Information from provider

ProviderSantiago Lamas
Provider affiliationProcesos Fisiológicos y Patológicos, Centro de Biología Molecular Severo Ochoa
Genetic informationPax8-rtTA cassette (Chr 8): expression of rtTA in renal proximal epithelial cells. TRE-CPT1A-P2A-GFP cassette (Col1a1 locus): CPT1A and GFP coding sequence under the control of the TRE element. P2A allows the protein excision among CPT1A and GFP.
Phenotypic informationHomozygous:
Phenotypically undistinguishable from wild-type mice. No anatomical anomalies, physiological variations, behavioural abnormalities or food uptake alterations.

Heterozygous:
No phenotypic alterations. Mice present a CPT1A overexpression and enhanced fatty acid oxidation in the tubular cells in the kidney.
Breeding historyPax8-rtTA mice (C57BL/6J) (JAX number 007176) were crossed with the newly generated TRE-CPT1A mice (B6N;129/Sv background). Resulting mice were backcrossed to C57BL/6J mice for at least 5 generations to generate this congenic strain.
References
  • Renal tubule Cpt1a overexpression protects from kidney fibrosis by restoring mitochondrial homeostasis.;Miguel Verónica, Tituaña Jessica, Herrero J Ignacio, Herrero Laura, Serra Dolors, Cuevas Paula, Barbas Coral, Puyol Diego Rodríguez, Márquez-Expósito Laura, Ruiz-Ortega Marta, Castillo Carolina, Sheng Xin, Susztak Katalin, Ruiz-Canela Miguel, Salas-Salvadó Jordi, González Miguel A Martínez, Ortega Sagrario, Ramos Ricardo, Lamas Santiago, ;2021;The Journal of clinical investigation;131;; 33465052
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6J

Literature references

  • Renal tubule Cpt1a overexpression protects from kidney fibrosis by restoring mitochondrial homeostasis.;Miguel Verónica, Tituaña Jessica, Herrero J Ignacio, Herrero Laura, Serra Dolors, Cuevas Paula, Barbas Coral, Puyol Diego Rodríguez, Márquez-Expósito Laura, Ruiz-Ortega Marta, Castillo Carolina, Sheng Xin, Susztak Katalin, Ruiz-Canela Miguel, Salas-Salvadó Jordi, González Miguel A Martínez, Ortega Sagrario, Ramos Ricardo, Lamas Santiago, ;2021;The Journal of clinical investigation;131;; 33465052

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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