R26-rtTAtg/0:tetO-Cpt1atg/0 (R26rtTA-CPT1A)

Status

Available to order

EMMA IDEM:13349
International strain nameR26-rtTAtg/0:tetO-Cpt1atg/0 (R26rtTA-CPT1A)
Alternative nameR26-rtTAtg/0:tetO-Cpt1atg/0 (R26rtTA-CPT1A)
Strain typeTransgenic Strains
Allele/Transgene symbolTRE-CPT1A
Gene/Transgene symbolTg(tetO-Cpt1a,-EGFP)

Information from provider

ProviderSantiago Lamas
Provider affiliationProcesos Fisiológicos y Patológicos, Centro de Biología Molecular Severo Ochoa
Genetic informationR26-rtTA cassette (Rosa 26 locus): ubiquitous expression of rtTA. TRE-CPT1A-P2A-GFP cassette (Col1a1 locus): CPT1A and GFP coding sequence under the control of the TRE element. P2A allows the protein excision among CPT1A and GFP.
Phenotypic informationHomozygous:
Phenotypically undistinguishable from wild-type mice. No anatomical anomalies, physiological variations, behavioural abnormalities or food uptake alterations.

Heterozygous:
No phenotypic alterations. Mice present a CPT1A overexpression and enhanced fatty acid oxidation ubiquitous.
Breeding historyR26-rtTA: tetO-CPT1A mice (B6N;129Sv background) were backcrossed to C57BL/6J mice for at least 5 generations to generate this congenic strain.
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6J

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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