C57BL/6N-Tg(Cxcl13-cre,-tdTomato)723Biat/Biat
| Status | Available to order |
| EMMA ID | EM:13610 |
| International strain name | C57BL/6N-Tg(Cxcl13-cre,-tdTomato)723Biat/Biat |
| Alternative name | C57BL/6N-Tg(Cxcl13-Cre/Tdtomato)723Biat |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Cxcl13-cre,-tdTomato)723Biat |
| Gene/Transgene symbol | Tg(Cxcl13-cre,-tdTomato)723Biat |
Information from provider
| Provider | Burkhard Ludewig |
| Provider affiliation | Institute of Immunobiology, Kantonsspital St.Gallen |
| Genetic information | C57BL/6N-Tg(Cxcl13-cre,-tdTomato)723Biat mice were generated using BAC RP23-57A17 (Invitrogen). Transgene integration was achieved using Red/ET recombination technologies (Gene bridges) according to the manufacturer’s protocol and as previously described (Sparwasser et al., PMID:14755803). The coding sequence for the Cre-recombinase and the reporter tdTomato, separated by an IRES sequence and including a stop codon, was inserted into Cxcl13 exon 1 using the endogenous ATG start codon. Proper transgene integration was assessed by Southern blot analysis and mice were genotyped using the following primers: forward 5'-TCTCTGCCCAGAGTCATCCT-3', reverse 5'-ATGCTCCTGTCTGTGTGCAG-3'. |
| Phenotypic information | Homozygous:Mice are kept heterozygous in order to avoid germline transmission.Heterozygous:Cre-recombinase and tdTomato are expressed under the control of the Cxcl13 promoter. |
| Breeding history | Mice are bred on C57BL/6 for N>10 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Literature references
- Remodeling of light and dark zone follicular dendritic cells governs germinal center responses.;Pikor Natalia B, Mörbe Urs, Lütge Mechthild, Gil-Cruz Cristina, Perez-Shibayama Christian, Novkovic Mario, Cheng Hung-Wei, Nombela-Arrieta César, Nagasawa Takashi, Linterman Michelle A, Onder Lucas, Ludewig Burkhard, ;2020;Nature immunology;21;649-659; 32424359
- Lymphatic Endothelial Cells Control Initiation of Lymph Node Organogenesis.;Onder Lucas, Mörbe Urs, Pikor Natalia, Novkovic Mario, Cheng Hung-Wei, Hehlgans Thomas, Pfeffer Klaus, Becher Burkhard, Waisman Ari, Rülicke Thomas, Gommerman Jennifer, Mueller Christopher G, Sawa Shinichiro, Scandella Elke, Ludewig Burkhard, ;2017;Immunity;47;80-92.e4; 28709801