CD1;B6N-Igs37tm2(ACTB-tdTomato,-EGFP)Biat/Biat

Status

Available to order

EMMA IDEM:14715
International strain nameCD1;B6N-Igs37tm2(ACTB-tdTomato,-EGFP)Biat/Biat
Alternative nameCD1;B6N-MADM-TG; Igs37; MADM-16-TG
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolIgs37tm2(ACTB-tdTomato, -EGFP)Biat
Gene/Transgene symbolIgs37

Information from provider

ProviderSimon Hippenmeyer
Provider affiliationInstitute of Science and Technology Austria (IST Austria)
Genetic informationThe TG MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an FRT site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the "Hipp16" locus on chromosome 16 (between Gm5767 and Usp7). (PMID 34161767)
Phenotypic informationHomozygous:
not available

Heterozygous:
not available
Breeding historyChimeras were generated by injecting C57BL/6N embryonic stem cells into BALB/cRj blastocysts and then crossed with CD1 mice. Afterwards they were intercrossed.
References
  • A genome-wide library of MADM mice for single-cell genetic mosaic analysis.;Contreras Ximena, Amberg Nicole, Davaatseren Amarbayasgalan, Hansen Andi H, Sonntag Johanna, Andersen Lill, Bernthaler Tina, Streicher Carmen, Heger Anna, Johnson Randy L, Schwarz Lindsay A, Luo Liqun, Rülicke Thomas, Hippenmeyer Simon, ;2021;Cell reports;35;109274; 34161767
  • Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance.;Hippenmeyer Simon, Johnson Randy L, Luo Liqun, ;2013;Cell reports;3;960-7; 23453967
  • Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration.;Hippenmeyer Simon, Youn Yong Ha, Moon Hyang Mi, Miyamichi Kazunari, Zong Hui, Wynshaw-Boris Anthony, Luo Liqun, ;2010;Neuron;68;695-709; 21092859
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria

Literature references

  • A genome-wide library of MADM mice for single-cell genetic mosaic analysis.;Contreras Ximena, Amberg Nicole, Davaatseren Amarbayasgalan, Hansen Andi H, Sonntag Johanna, Andersen Lill, Bernthaler Tina, Streicher Carmen, Heger Anna, Johnson Randy L, Schwarz Lindsay A, Luo Liqun, Rülicke Thomas, Hippenmeyer Simon, ;2021;Cell reports;35;109274; 34161767
  • Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance.;Hippenmeyer Simon, Johnson Randy L, Luo Liqun, ;2013;Cell reports;3;960-7; 23453967
  • Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration.;Hippenmeyer Simon, Youn Yong Ha, Moon Hyang Mi, Miyamichi Kazunari, Zong Hui, Wynshaw-Boris Anthony, Luo Liqun, ;2010;Neuron;68;695-709; 21092859

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Availabilities

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Practical information

Genotyping protocol

Example health report
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Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
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