B6J;B6N-Nkx1-2tm2296.1(cre/ERT2)Arte/H
| Status | Available to order |
| EMMA ID | EM:15076 |
| International strain name | B6J;B6N-Nkx1-2tm2296.1(cre/ERT2)Arte/H |
| Alternative name | Nkx1-2CreERT2 |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Nkx1-2tm2296.1(cre/ERT2)Arte, |
| Gene/Transgene symbol | Nkx1-2 |
Information from provider
| Provider | James Briscoe |
| Provider affiliation | The Francis Crick Institute |
| Genetic information | In this mouse line CreERT2 recombinase is driven under the control of the endogenous promoter of the transcription factor Nkx1-2. The targeting strategy was designed to replace Nkx1-2 exon 1 and the splice donor site at the junction between exon 1 and intron 1 with a cassette containing the open reading frame of CreERT2 (see Rodrigo-Albors et al. Development (2018) 145, dev164319. doi:10.1242/dev.164319). |
| Phenotypic information | Homozygous:CreERT2 will be expressed from the endogenous Nkx1-2 promoter and should thus recapitulate the expression pattern of the Nkx1-2 gene. The replacement of the Nkx1-2 coding sequence in exon 1 with the CreERT2 and the polyadenylation signal should result in the loss of function of the Nkx1-2 gene. However, loss of function of the Nkx1-2 (aka Sax1) gene did not generate a phenotype in either heterozygous or homozygous mice. This is likely due to genetic compensation or functional redundancy by another related gene/s and confirms previous findings, see Schubert, F. R., Fainsod, A., Gruenbaum, Y. and Gruss, P. (1995). Expression of the novel murine homeobox gene Sax-1 in the developing nervous system. Mech. Dev. 51, 99-114.Heterozygous:Consistent with the Schubert et al 1995 study; no phenotype has been observed with this reporter line. |
| Breeding history | Taconic provided two breeding pairs of heterozygous C57BL/6–Nkx1-2tm2296(Cre-ER(T2))Arte mice. These mice carried a puromycin-expressing cassette flanked by F3 sites, which was removed upon crossing to Flp-expressing mice. The resulting mice express CreERT2 from the endogenous Nkx1-2 promoter (Nkx1-2CreERT2). Nkx1-2CreERT2 mice were then bred to homozygosity to establish a breeding colony. We maintained this homozygous Nkx1-2CreERT2 colony for more than nine generations without any obvious deleterious effects. Genetic background: C57BL/6NTac and C57BL/6J mixed. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Lineage tracing of axial progenitors using Nkx1-2CreERT2 mice defines their trunk and tail contributions.;Rodrigo Albors Aida, Halley Pamela A, Storey Kate G, ;2018;Development (Cambridge, England);145;; 30201686