NOD.129-Rag1tm1Bal B2mtm1Unc H2-Ab1tm1Doi/Orl

Status

Available to order

EMMA IDEM:01638
International strain nameNOD.129-Rag1tm1Bal B2mtm1Unc H2-Ab1tm1Doi/Orl
Alternative nameNOD-IAbeta-beta2m-RAG1-/-
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolRag1tm1Bal,
Gene/Transgene symbolRag1

Information from provider

ProviderFrancoise Lepault
Provider affiliationINSERM U561 - Hopital Saint Vincent de Paul
Genetic informationNOD mice deficient for IAbeta (H2-Ab1), beta-2 microglobulin (B2m) and Rag1 molecules.
Phenotypic informationThese mice have no T, B, NKT lymphocytes and no MHC molecules. Do not develop diabetes. Breeding more difficult than with wild-type mice.
Breeding historyNOD mice deficient for IAbeta were crossed with NOD mice deficient for beta2m to generate NOD mice deficient for both molecules. The latter mice were then crossed with NOD mice deficient for Rag1. The three NOD knock-out strains were used after 10 backcrosses to the NOD background.
References
  • Mice lacking MHC class II molecules.;Cosgrove D, Gray D, Dierich A, Kaufman J, Lemeur M, Benoist C, Mathis D, ;1991;Cell;66;1051-66; 1909605
  • Major histocompatibility complex class I molecules are required for the development of insulitis in non-obese diabetic mice.;Katz J, Benoist C, Mathis D, ;1993;European journal of immunology;23;3358-60; 8258349
  • RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination.;Oettinger M A, Schatz D G, Gorka C, Baltimore D, ;1990;Science (New York, N.Y.);248;1517-23; 2360047

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • decreased level of surface class I molecules / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • decreased IgG level / MGI
  • abnormal pancreas morphology / MGI
  • decreased susceptibility to bacterial infection / MGI
  • increased IgM level / MGI
  • decreased susceptibility to autoimmune diabetes / MGI
  • decreased circulating serum albumin level / MGI
  • increased T-helper 1 cell number / MGI
  • abnormal interleukin level / MGI
  • abnormal tumor necrosis factor level / MGI
  • increased susceptibility to infection induced morbidity/mortality / MGI
  • absent CD8-positive, alpha-beta T cells / MGI
  • abnormal pancreatic beta cell morphology / MGI
  • abnormal T cell number / MGI
  • insulitis / MGI
  • abnormal neuron proliferation / MGI
  • abnormal immune system morphology / MGI
  • abnormal spleen morphology / MGI
  • abnormal immune system cell morphology / MGI
  • postnatal growth retardation / MGI
  • abnormal immune system physiology / MGI
  • reduced fertility / MGI
  • abnormal spleen periarteriolar lymphoid sheath morphology / MGI
  • abnormal spleen marginal zone morphology / MGI
  • abnormal CD4-positive, alpha beta T cell morphology / MGI
  • abnormal T cell physiology / MGI
  • abnormal B cell physiology / MGI
  • abnormal immune system organ morphology / MGI
  • abnormal cytokine secretion / MGI
  • decreased susceptibility to parasitic infection / MGI
  • increased susceptibility to parasitic infection / MGI
  • abnormal CD4-positive, alpha-beta T cell physiology / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • abnormal spleen B cell follicle morphology / MGI
  • absent spleen germinal center / MGI
  • decreased IgG1 level / MGI
  • abnormal lymph node cell ratio / MGI

Literature references

  • Mice lacking MHC class II molecules.;Cosgrove D, Gray D, Dierich A, Kaufman J, Lemeur M, Benoist C, Mathis D, ;1991;Cell;66;1051-66; 1909605
  • Major histocompatibility complex class I molecules are required for the development of insulitis in non-obese diabetic mice.;Katz J, Benoist C, Mathis D, ;1993;European journal of immunology;23;3358-60; 8258349
  • RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination.;Oettinger M A, Schatz D G, Gorka C, Baltimore D, ;1990;Science (New York, N.Y.);248;1517-23; 2360047

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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