B6.Cg-Rag2tm1Fwa Tyrc Foxn1nu/FwaOrl

Status

Available to order

EMMA IDEM:00164
International strain nameB6.Cg-Rag2tm1Fwa Tyrc Foxn1nu/FwaOrl
Alternative nameRag2°-nu
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolRag2tm1Fwa,
Gene/Transgene symbolRag2

Information from provider

ProviderFrederick Alt
Provider affiliationNational Cancer Institute
Genetic informationB6-Rag2: mutant mice deficient in the Rag2 gene; obtained by mutation of the Rag2 gene by homologous recombination (insertion of the PMC1neo gene into the unique exon of Rag2 gene, leading to the deletion of 286 amino acids); nu (nude): forkhead box N1 nude mutation (Foxn1nu).
Phenotypic informationRag2 knock-out: homozygous mutants are viable even if they are smaller in size than wild-type or heterozygous littermates; homozygous animals do not produce mature B or T lymphocytes; deletion of a large portion of the Rag2 gene produces a total inability to initiate V(D)J rearrangement, leading to a severe combined immune deficiency (SCID). Foxn1nu (nude): hairlessness, athymia and T-cell deficiency (immunodeficiency). We can provide only B6-Rag2 males and nu/+ females and the customer should have to do the breeding.
References
  • RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • small thymus / MGI
  • abnormal thymus morphology / MGI
  • increased incidence of corneal inflammation / MGI
  • increased susceptibility to viral infection / MGI
  • blepharitis / MGI
  • corneal vascularization / MGI
  • abnormal nail morphology / MGI
  • nail dystrophy / MGI
  • abnormal nail plate morphology / MGI
  • abnormal nail matrix morphology / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased leukocyte cell number / MGI
  • abnormal liver morphology / MGI
  • abnormal spleen morphology / MGI
  • small spleen / MGI
  • athymia / MGI
  • small ovary / MGI
  • thin skin / MGI
  • decreased body weight / MGI
  • decreased body size / MGI
  • wavy vibrissae / MGI
  • short vibrissae / MGI
  • absent vibrissae / MGI
  • postnatal growth retardation / MGI
  • reduced male fertility / MGI
  • female infertility / MGI
  • abnormal estrous cycle / MGI
  • premature death / MGI
  • abnormal lymph node T cell domain morphology / MGI
  • abnormal Peyer's patch germinal center morphology / MGI
  • asthenozoospermia / MGI
  • hairless / MGI
  • abnormal venule morphology / MGI
  • abnormal splenic cell ratio / MGI
  • coiled sperm flagellum / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • impaired spatial learning / MGI
  • increased bone mineral density / MGI
  • abnormal trabecular bone morphology / MGI
  • abnormal compact bone morphology / MGI
  • abnormal coat/ hair morphology / MGI
  • abnormal hair follicle morphology / MGI
  • deformed nails / MGI
  • abnormal thymus development / MGI
  • abnormal epidermal layer morphology / MGI
  • abnormal epidermis stratum basale morphology / MGI
  • abnormal epidermis stratum granulosum morphology / MGI
  • abnormal epidermis stratum corneum morphology / MGI
  • abnormal embryonic tissue morphology / MGI
  • abnormal hair shaft morphology / MGI
  • abnormal hair cuticle / MGI
  • abnormal hair cortex morphology / MGI
  • endocrine/exocrine gland phenotype / MGI
  • abnormal cutaneous collagen fibril morphology / MGI
  • reduced hair shaft melanin granule number / MGI
  • abnormal hair cortex keratinization / MGI
  • abnormal hair follicle inner root sheath morphology / MGI
  • short nails / MGI
  • decreased susceptibility to bacterial infection / MGI
  • decreased circulating alanine transaminase level / MGI
  • ascites / MGI
  • decreased circulating tumor necrosis factor level / MGI
  • decreased interferon-gamma secretion / MGI
  • decreased circulating interleukin-6 level / MGI
  • decreased circulating interleukin-2 level / MGI
  • chromosomal instability / MGI
  • increased susceptibility to viral infection induced morbidity/mortality / MGI
  • abnormal circulating cytokine level / MGI
  • increased plasmacytoma incidence / MGI
  • decreased circulating luteinizing hormone level / MGI
  • decreased circulating follicle stimulating hormone level / MGI
  • abnormal circulating gonadotropin level / MGI
  • abnormal T cell activation / MGI
  • abnormal lymph node morphology / MGI
  • increased length of allograft survival / MGI
  • decreased lymphocyte cell number / MGI
  • decreased T cell number / MGI
  • abnormal spleen physiology / MGI
  • abnormal muscle morphology / MGI
  • decreased skeletal muscle mass / MGI
  • decreased skeletal muscle fiber size / MGI
  • increased granulocyte number / MGI
  • spleen hypoplasia / MGI
  • arrested B cell differentiation / MGI
  • decreased IgM level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • increased susceptibility to infection / MGI
  • abnormal effector T cell morphology / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • decreased double-positive T cell number / MGI
  • increased macrophage cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • absent mature B cells / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • decreased thymocyte number / MGI
  • lung inflammation / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal macrophage physiology / MGI
  • colitis / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal response to transplant / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal B cell morphology / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal stomach mucosa morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal B cell number / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI

Literature references

  • RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487

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Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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Practical information

Genotyping protocol

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