C.129P2-Krt5tm1Tmm/Ieg

Status

Available to order

EMMA IDEM:01740
International strain nameC.129P2-Krt5tm1Tmm/Ieg
Alternative nameK5(+/-) heterozygous
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolKrt5tm1Tmm,
Gene/Transgene symbolKrt5

Information from provider

ProviderThomas Magin
Provider affiliationInstitute of physiological chemistry / Dept. of cellular biochemistry, University of Bonn
Genetic informationThe promoter and first 2 exons of keratin 5 gene (Krt5) were replaced with an Hprt cassette via homologous recombination in ES cells.
Phenotypic informationHeterozygous pups appeared inconspicuous at birth and displayed no overt phenotype. Homozygotes were easily identified at birth because they displayed an extremely delicate, loose, and fragile epidermis that lost contact with the dermis. Paw areas were denuded in some pups. The pups appeared to have breathing problems because sometimes the torn off epidermis had moved to close their nostrils and mouth. Stomachs of homozygous pups contained no milk. All Krt5 -/- animals died within the first hour after birth. Obviously, the structural weakening of the epidermis caused by the loss of Krt5 rendered the epidermis extremely fragile and prone to rupturing during the physical trauma of birth.
Breeding historyK5 mice have been backcrossed to the BALB/c background for 8 generations.
References
  • Complete cytolysis and neonatal lethality in keratin 5 knockout mice reveal its fundamental role in skin integrity and in epidermolysis bullosa simplex.;Peters B, Kirfel J, Büssow H, Vidal M, Magin T M, ;2001;Molecular biology of the cell;12;1775-89; 11408584

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • abnormal esophageal epithelium morphology / MGI
  • abnormal stomach morphology / MGI
  • abnormal autopod morphology / MGI
  • abnormal tongue epithelium morphology / MGI
  • abnormal epidermal layer morphology / MGI
  • abnormal tail morphology / MGI
  • abnormal palate morphology / MGI
  • absent gastric milk in neonates / MGI
  • neonatal lethality, complete penetrance / MGI

Literature references

  • Complete cytolysis and neonatal lethality in keratin 5 knockout mice reveal its fundamental role in skin integrity and in epidermolysis bullosa simplex.;Peters B, Kirfel J, Büssow H, Vidal M, Magin T M, ;2001;Molecular biology of the cell;12;1775-89; 11408584

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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