CD1(B6)-Tg(Hoxb1-cre)r4Mist/Cnrm

Status

Available to order

EMMA IDEM:01796
International strain nameCD1(B6)-Tg(Hoxb1-cre)r4Mist/Cnrm
Alternative nameR4-CRE
Strain typeTransgenic Strains
Allele/Transgene symbolTg(Hoxb1-cre)r4Mist,
Gene/Transgene symbolTg(Hoxb1-cre)r4Mist

Information from provider

ProviderMichèle Studer
Provider affiliationTIGEM (Telethon Institute of Genetics and Medicine)
Genetic informationGeneration of the rhombomere (r)4-cre transgenic line: A 2,7 kb XhoI-HindIII construct containing the cre recombinase gene and the metallothionein-1 polyadenylation signal obtained from pBS185 (GibcoBRL, Cat# 10347-011) was cloned into EcoRV -SacII of pWexP3β (Danielian and McMahon 1996; gift from Paul S. Danielian), which includes a Wnt1 basic promoter. The 741 bp ApaI-SpeI fragment containing the Hoxb1 r3/r5 repressor and the 1043 bp SpeI-SmaI fragment containing the Hoxb1 5' r4 enhancer were cloned inverted (Studer et al. 1994) and inserted into the BglII of the above plasmid. This combination leads to restricted expression of cre recombinase in rhombomere 4 of developing embryo hindbrain. The targeting construct was digested with NotI and injected into pronuclei of CD1 oocytes.
Phenotypic informationNone.
Breeding historyThe strain was maintained on a C57BL/6 background.
References
  • Role of a conserved retinoic acid response element in rhombomere restriction of Hoxb-1.;Studer M, Pöpperl H, Marshall H, Kuroiwa A, Krumlauf R, ;1994;Science (New York, N.Y.);265;1728-32; 7916164

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy
Breeding at archiving centreOriginal background CD1, then backcrossed to B6 and further backcrossed less than 3 times to CD1

Literature references

  • Role of a conserved retinoic acid response element in rhombomere restriction of Hoxb-1.;Studer M, Pöpperl H, Marshall H, Kuroiwa A, Krumlauf R, ;1994;Science (New York, N.Y.);265;1728-32; 7916164

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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