MGI phenotypes (allele matching)
- immune system phenotype / MGI
B6.129S2-Lime1tm1Mal/Orl
| Status | Available to order |
| EMMA ID | EM:01815 |
| International strain name | B6.129S2-Lime1tm1Mal/Orl |
| Alternative name | LIME KO |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Lime1tm1Mal, |
| Gene/Transgene symbol | Lime1 |
Information from provider
| Provider | Malissen Marie |
| Provider affiliation | Centre d'Immunologi de Marseille Luminy |
| Genetic information | Complete knock-out with deletion of exons 1-5. Deletion was obtained by the introduction of a loxP-neo cassette which was subsequently removed by crossing with cre recombinase deleter mice. |
| Phenotypic information | Lime1 (or Lime, Lck interacting molecule) is predominantly expressed in T lymphocytes. Lime becomes phosphorylated after coreceptors cross-linking. Lime appears to be involved in regulation of T cell activation by coreceptors. The Lime1 knock-out mutation does not prevent normal development and activation of lymphocytes. |
| Breeding history | This strain was first crossed onto a cre-deleter strain, followed by 8 backcrosses to C57BL/6. |
| References |
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Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Disease and phenotype information
Literature references
- LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.;Brdicková Nadezda, Brdicka Tomás, Angelisová Pavla, Horváth Ondrej, Spicka Jiri, Hilgert Ivan, Paces Jan, Simeoni Luca, Kliche Stefanie, Merten Camilla, Schraven Burkhart, Horejsí Václav, ;2003;The Journal of experimental medicine;198;1453-62; 14610046
- LIME acts as a transmembrane adapter mediating BCR-dependent B-cell activation.;Ahn Eunseon, Lee Hyunsook, Yun Yungdae, ;2006;Blood;107;1521-7; 16249387
- LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation.;Hur Eun Mi, Son Myoungsun, Lee Ok-Hee, Choi Young Bong, Park Changwon, Lee Hyunsook, Yun Yungdae, ;2003;The Journal of experimental medicine;198;1463-73; 14610044
- Deletion of the LIME adaptor protein minimally affects T and B cell development and function.;Grégoire Claude, Simova Sarka, Wang Ying, Sansoni Amandine, Richelme Sylvie, Schmidt-Giese Anja, Simeoni Luca, Angelisova Pavla, Reinhold Dirk, Schraven Burkhart, Horejsi Vaclav, Malissen Bernard, Malissen Marie, ;2007;European journal of immunology;37;3259-69; 17918199