B6;129P2-Polmtm1Crey/Orl

Status

Available to order

EMMA IDEM:01972
International strain nameB6;129P2-Polmtm1Crey/Orl
Alternative nameDNA polymerase mu knockouts
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolPolmtm1Crey,
Gene/Transgene symbolPolm

Information from provider

ProviderBarbara Bertocci
Provider affiliationINSERM 783 ex373
Genetic informationThe region of mu DNA polymerase gene (Polm) between exons 6 and 10 was deleted and replaced by neomycin, which is flanked by two loxP sites.
Phenotypic informationPeripheral B cell deficiency, impaired production of bone marrow, immature B cells and defects in immunoglobulin light chain rearrangement.
References
  • Cutting edge: DNA polymerases mu and lambda are dispensable for Ig gene hypermutation.;Bertocci Barbara, De Smet Annie, Flatter Eric, Dahan Auriel, Bories Jean-Christophe, Landreau Catherine, Weill Jean-Claude, Reynaud Claude-Agnès, ;2002;Journal of immunology (Baltimore, Md. : 1950);168;3702-6; 11937519
  • Immunoglobulin kappa light chain gene rearrangement is impaired in mice deficient for DNA polymerase mu.;Bertocci Barbara, De Smet Annie, Berek Claudia, Weill Jean-Claude, Reynaud Claude-Agnès, ;2003;Immunity;19;203-11; 12932354

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal immunoglobulin light chain V-J recombination / MGI
  • abnormal Peyer's patch morphology / MGI
  • lymphoid hypoplasia / MGI
  • decreased B cell number / MGI
  • abnormal B cell proliferation / MGI
  • immune system phenotype / MGI
  • reproductive system phenotype / MGI

Literature references

  • Cutting edge: DNA polymerases mu and lambda are dispensable for Ig gene hypermutation.;Bertocci Barbara, De Smet Annie, Flatter Eric, Dahan Auriel, Bories Jean-Christophe, Landreau Catherine, Weill Jean-Claude, Reynaud Claude-Agnès, ;2002;Journal of immunology (Baltimore, Md. : 1950);168;3702-6; 11937519
  • Immunoglobulin kappa light chain gene rearrangement is impaired in mice deficient for DNA polymerase mu.;Bertocci Barbara, De Smet Annie, Berek Claudia, Weill Jean-Claude, Reynaud Claude-Agnès, ;2003;Immunity;19;203-11; 12932354

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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