B6Rcc.129-Cd44tm1.1Ugu Spp1tm1Rit/Cnrm

Status

Available to order

EMMA IDEM:01990
International strain nameB6Rcc.129-Cd44tm1.1Ugu Spp1tm1Rit/Cnrm
Alternative nameC57 BL/6RCC CD44v6v7-/- OPN-/-
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolSpp1tm1Rit,
Gene/Transgene symbolSpp1

Information from provider

ProviderUrsula Günthert
Provider affiliationInstitut für Pathologie, University of Basel
Genetic informationA loxP site was inserted 3' of exon v6 and a neomycin resistance cassette, with a 5' loxP site, was inserted 5' of exon v6 of the Cd44 gene. During insertion, 1.6 kb of sequence was lost in the exon v7 region. Cre recombinase was transiently expressed in ES cells allowing the removal of neomycin cassette and exon v6. Mice lack both exon v6 and v7. The neomycin cassette from pMC1neo was inserted into the EagI site in exon 6 of the Spp1 (secreted phosphoprotein 1; formerly: Opn, osteopontin) gene (a 4.8 kb fragment from 129 mice), that had been cloned into pBluescript (Rittling et al., 1998).
Phenotypic informationCd44v6v7-/-: Strongly reduced symptoms in autoimmune diseases and haemopoiesis. Opn (Spp1)-/-: Strongly reduced symptoms in autoimmune diseases and impaired bone remodeling.
Breeding history10 generations backcrossed onto C57BL/6Rcc; currently bred as C57BL/6Rcc Cd44v6v7-/- Opn-/-.
References
  • Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
  • The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease.;Chabas D, Baranzini S E, Mitchell D, Bernard C C, Rittling S R, Denhardt D T, Sobel R A, Lock C, Karpuj M, Pedotti R, Heller R, Oksenberg J R, Steinman L, ;2001;Science (New York, N.Y.);294;1731-5; 11721059
  • Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro.;Rittling S R, Matsumoto H N, McKee M D, Nanci A, An X R, Novick K E, Kowalski A J, Noda M, Denhardt D T, ;1998;Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research;13;1101-11; 9661074
  • Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival.;Denhardt D T, Noda M, O'Regan A W, Pavlin D, Berman J S, ;2001;The Journal of clinical investigation;107;1055-61; 11342566

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • decreased T cell proliferation / MGI
  • abnormal digestive system physiology / MGI
  • immune system phenotype / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal T-helper 2 physiology / MGI
  • abnormal liver morphology / MGI
  • decreased acute inflammation / MGI
  • decreased tumor necrosis factor secretion / MGI
  • decreased interferon-gamma secretion / MGI
  • decreased physiological sensitivity to xenobiotic / MGI
  • increased bone mineral density / MGI
  • abnormal osteoclast physiology / MGI
  • abnormal cardiovascular system physiology / MGI
  • abnormal inflammatory response / MGI
  • decreased susceptibility to viral infection / MGI
  • dilated renal tubules / MGI
  • decreased heart weight / MGI
  • delayed wound healing / MGI
  • nervous system phenotype / MGI
  • abnormal physiological neovascularization / MGI
  • renal tubular necrosis / MGI
  • increased osteoclast cell number / MGI
  • decreased osteoclast cell number / MGI
  • decreased bone resorption / MGI
  • abnormal osteoblast physiology / MGI
  • abnormal response to infection / MGI
  • decreased cardiac muscle contractility / MGI
  • increased susceptibility to injury / MGI
  • skeleton phenotype / MGI
  • hyporesponsive to tactile stimuli / MGI
  • increased circulating creatinine level / MGI
  • increased blood urea nitrogen level / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • increased interleukin-10 secretion / MGI
  • decreased interleukin-12 secretion / MGI
  • increased interleukin-4 secretion / MGI
  • decreased sensitivity to induced cell death / MGI
  • increased trabecular bone thickness / MGI
  • mortality/aging / MGI
  • decreased fibroblast chemotaxis / MGI
  • increased bone marrow cell number / MGI
  • abnormal blood cell morphology/development / MGI
  • abnormal bone mineralization / MGI
  • increased hematopoietic stem cell number / MGI
  • increased sensitivity to induced morbidity/mortality / MGI

Literature references

  • Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
  • The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease.;Chabas D, Baranzini S E, Mitchell D, Bernard C C, Rittling S R, Denhardt D T, Sobel R A, Lock C, Karpuj M, Pedotti R, Heller R, Oksenberg J R, Steinman L, ;2001;Science (New York, N.Y.);294;1731-5; 11721059
  • Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro.;Rittling S R, Matsumoto H N, McKee M D, Nanci A, An X R, Novick K E, Kowalski A J, Noda M, Denhardt D T, ;1998;Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research;13;1101-11; 9661074
  • Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival.;Denhardt D T, Noda M, O'Regan A W, Pavlin D, Berman J S, ;2001;The Journal of clinical investigation;107;1055-61; 11342566

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

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