B6Rcc.Cg-Cd44tm1.1Ugu ApcMin/Cnrm

Status

Available to order

EMMA IDEM:02001
International strain nameB6Rcc.Cg-Cd44tm1.1Ugu ApcMin/Cnrm
Alternative nameC57 BL/6RCC Min/+ CD44v6v7-/-
Strain typeInduced Mutant Strains : Chemically-induced
Allele/Transgene symbolApcMin,
Gene/Transgene symbolApc

Information from provider

ProviderUrsula Günthert
Provider affiliationInstitut für Pathologie, University of Basel
Genetic informationEthylnitrosurea induced mutation. A transversion point mutation in the Apc (adenomatosis polyposis coli) gene that alters nucleotide 2549 from a T to an A and converts codon 850 from one encoding a leucine to a stop codon, generating the mutant allele ApcMin(adenomatosis polyposis coli; multiple intestinal neoplasia). A loxP site was inserted 3' of exon v6 and a neomycin resistance cassette, with a 5' loxP site, was inserted 5' of exon v6 of the Cd44 gene. During insertion, 1.6 kb of sequence was lost in the exon v7 region; cre recombinase was transiently expressed in ES cells allowing the removal of neomycin cassette and exon v6. Mice lack both exon v6 and v7.
Phenotypic informationMin/+: Development of early stages of colon carcinoma: polyps. Cd44v6v7-/-: Expression of Cd44v6 is an independent prognostic factor for a poor prognosis in colon carcinoma. Expression of Cd44v6 isoforms has been shown to be an independent prognostic factor for a poor prognosis in human colon carcinoma (Mulder et al., 1994). To determine whether and to what extent Cd44v6/v7-containing isoforms are involved in the early stages of adenoma formation, we crossbred ApcMin mice with mice bearing deletions for Cd44v6/v7. While Min/+ x Cd44v6/v7 wild-type mice developed on the average more than 50 adenomas at an age of 12-18 weeks, Min/+ x Cd44v6/v7 knock-out mice developed fewer than 10 adenomas, and many had no polyps at all. Moreover, the life span of Min/+ x Cd44v6/v7 null mice increased substantially to more than 60 weeks (p
Breeding history10 generations backcrossed onto C57BL/6Rcc; currently maintained as C57BL/6Rcc Min/+ Cd44v6v7-/-.
References
  • Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
  • A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.;Moser A R, Pitot H C, Dove W F, ;1990;Science (New York, N.Y.);247;322-4; 2296722

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • abnormal mammary gland development / MGI
  • abnormal ovarian folliculogenesis / MGI
  • abnormal ovarian follicle morphology / MGI
  • absent mature ovarian follicles / MGI
  • absent corpus luteum / MGI
  • abnormal vagina epithelium morphology / MGI
  • decreased body weight / MGI
  • increased incidence of induced tumors / MGI
  • ovary atrophy / MGI
  • uterus atrophy / MGI
  • reproductive system phenotype / MGI
  • adrenal gland hyperplasia / MGI
  • absent nipple / MGI
  • increased mammary gland tumor incidence / MGI
  • mammary gland hyperplasia / MGI
  • anemia / MGI
  • premature death / MGI
  • increased intestinal adenoma incidence / MGI
  • increased intestinal adenocarcinoma incidence / MGI
  • abnormal pregnancy / MGI
  • extended life span / MGI
  • aneuploidy / MGI
  • increased incidence of tumors by chemical induction / MGI
  • decreased intestinal adenoma incidence / MGI
  • decreased hematocrit / MGI
  • hyperlipidemia / MGI
  • melena / MGI
  • rectal prolapse / MGI
  • abnormal intestinal mucosa morphology / MGI
  • increased mammary adenocarcinoma incidence / MGI
  • intestinal obstruction / MGI
  • abnormal intestinal goblet cell morphology / MGI
  • abnormal large intestine crypts of Lieberkuhn morphology / MGI
  • intestine polyps / MGI
  • abnormal enterocyte proliferation / MGI
  • decreased T cell number / MGI
  • decreased NK cell number / MGI
  • decreased splenocyte number / MGI
  • neoplasm / MGI
  • increased circulating triglyceride level / MGI
  • increased circulating free fatty acid level / MGI
  • postnatal growth retardation / MGI
  • increased colonic adenoma incidence / MGI
  • hepatic steatosis / MGI
  • decreased macrophage cell number / MGI
  • increased prostaglandin level / MGI
  • abnormal embryo development / MGI
  • abnormal embryo size / MGI
  • abnormal egg cylinder morphology / MGI
  • abnormal cell physiology / MGI
  • embryonic lethality, complete penetrance / MGI
  • decreased embryo size / MGI
  • embryonic lethality between implantation and placentation / MGI
  • absent mandible / MGI
  • abnormal brain development / MGI
  • acrania / MGI
  • perinatal lethality, complete penetrance / MGI
  • lethality throughout fetal growth and development, incomplete penetrance / MGI
  • absent midbrain / MGI
  • absent forebrain / MGI
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • decreased T cell proliferation / MGI
  • abnormal digestive system physiology / MGI
  • immune system phenotype / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal T-helper 2 physiology / MGI

Literature references

  • Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
  • A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse.;Moser A R, Pitot H C, Dove W F, ;1990;Science (New York, N.Y.);247;322-4; 2296722

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
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Material Transfer Agreement (MTA)
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