- decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
- decreased T cell proliferation / MGI
- increased granulocyte number / MGI
- abnormal spleen morphology / MGI
- spleen hypoplasia / MGI
- decreased body size / MGI
- arrested B cell differentiation / MGI
- decreased IgM level / MGI
- thymus hypoplasia / MGI
- arrested T cell differentiation / MGI
- increased susceptibility to infection / MGI
- abnormal effector T cell morphology / MGI
- increased natural killer cell mediated cytotoxicity / MGI
- decreased double-positive T cell number / MGI
- increased macrophage cell number / MGI
- decreased CD4-positive, alpha beta T cell number / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- lymph node hypoplasia / MGI
- absent mature B cells / MGI
- abnormal T cell receptor V(D)J recombination / MGI
- abnormal immunoglobulin V(D)J recombination / MGI
- decreased thymocyte number / MGI
- lung inflammation / MGI
- increased susceptibility to bacterial infection / MGI
- abnormal macrophage physiology / MGI
- colitis / MGI
- abnormal T-helper 1 physiology / MGI
- abnormal response to transplant / MGI
- abnormal interferon secretion / MGI
- abnormal chemokine secretion / MGI
- decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
- abnormal intestinal mucosa morphology / MGI
- abnormal B cell morphology / MGI
- decreased susceptibility to parasitic infection / MGI
- decreased double-negative T cell number / MGI
- increased double-negative T cell number / MGI
- decreased susceptibility to type IV hypersensitivity reaction / MGI
- abnormal stomach mucosa morphology / MGI
- abnormal intestinal epithelium morphology / MGI
- abnormal B cell number / MGI
- decreased pre-B cell number / MGI
- decreased mature B cell number / MGI
- increased immature B cell number / MGI
- decreased immature B cell number / MGI
- abnormal gamma-delta T cell differentiation / MGI
CByIco.129-Rag2tm1Fwa Cd44tm2.1Ugu/H
Status | Available to order |
EMMA ID | EM:02021 |
International strain name | CByIco.129-Rag2tm1Fwa Cd44tm2.1Ugu/H |
Alternative name | BALB/cByJIco Rag2-/- CD44v10-/- |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Rag2tm1Fwa, |
Gene/Transgene symbol | Rag2 |
Information from provider
Provider | Ursula Günthert |
Provider affiliation | Institut für Pathologie, University of Basel |
Genetic information | The mouse Cd44 variant region was isolated from a 129SV genomic library. Two 34 bp loxP sites were inserted in direct repeats into a single BstEII site 5' of exon v10 and at the 3' end of the neo resistance cassette, which was then inserted into the single BstXI site 3' of exon v10. The targeting vector was transfected in ES cells and homologous recombinant clones injected into C57BL/6 blastocysts. Chimaeric male offspring was then mated with C57BL/6 cre recombinase deleter females to allow the removal of the loxP flanked region. Offspring was genotyped by PCR using exon v10 flanking oligos and analysing for the deletion of the loxP targeted region. Insertion of a neomycin resistance cassette into the unique exon of the Rag2 gene by homologous recombination leading to the deletion of 286 amino acids. |
Phenotypic information | Rag2-/-: No mature T and B cells. Cd44v10-/-: Strongly reduced symptoms in autoimmune diseases. |
Breeding history | Backcrossed for 10 generations; kept as homozygous strain on BALB/cByJIco background. |
References |
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Information from EMMA
Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Animals used for archiving | homozygous BALB/cByJIco, homozygous BALB/cByJIco |
Breeding at archiving centre | Maintained by intercrossing double homozygotes prior to, and during archiving |
Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Severe combined immunodeficiency due to complete RAG1/2 deficiency / Orphanet_331206
- Omenn syndrome / Orphanet_39041
- Combined immunodeficiency with granulomatosis / Orphanet_157949
MGI phenotypes (allele matching)
Literature references
- RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487
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