MGI phenotypes (allele matching)
CByIco.129(Cg)-Cd44tm2.1Ugu/Ieg
| Status | Available to order |
| EMMA ID | EM:02079 |
| International strain name | CByIco.129(Cg)-Cd44tm2.1Ugu/Ieg |
| Alternative name | BALB/cByJIco CD44v10-/- |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Cd44tm2.1Ugu, |
| Gene/Transgene symbol | Cd44 |
Information from provider
| Provider | Ursula Günthert |
| Provider affiliation | Institut für Pathologie, University of Basel |
| Genetic information | The mouse Cd44 variant region was isolated from a 129SV genomic library. Two 34 bp loxP sites were inserted in direct repeats into a single BstEII site 5' of exon v10 and at the 3' end of the neo resistance cassette, which was then inserted into the single BstXI site 3' of exon v10. The targeting vector was transfected in ES cells and homologous recombinant clones injected into C57BL/6 blastocysts. Chimaeric male offspring was then mated with C57BL/6 Cre deleter females to allow the removal of the loxP flanked region. Offspring was genotyped by PCR using exon v10 flanking oligos and analysing for the deletion of the loxP targeted region. |
| Phenotypic information | Cd44v10-/-: Strongly reduced symptoms in autoimmune diseases. |
| Breeding history | Backcrossed for 10 generations; kept as homozygous strain on BALB/cByJIco background. |
| References |
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Information from EMMA
| Archiving centre | Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany |
Disease and phenotype information
Literature references
- Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330