B6.129-Tgm2tm1Gml/Cnrm

Status

Available to order

EMMA IDEM:02283
International strain nameB6.129-Tgm2tm1Gml/Cnrm
Alternative nameTG2KO
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolTgm2tm1Gml,
Gene/Transgene symbolTgm2

Information from provider

ProviderGerry Melino
Provider affiliationUniversity of Tor Vergata
Genetic informationThe targeting vector was constructed by cloning an ~4-kb EcoRV/BamHI fragment containing the sequence from intron 6 to exon 9, into the pPNT vector, 3' of the neomycin resistance gene between the XbaI and BamHI unique sites. An ~2-kb fragment containing intron 3 was generated by genotyping using primers designed on the basis of the sequence of exons 4 and 5. This fragment was cloned into the XhoI site of the pPNT vector 5' of the neomycin resistance gene. This construct deletes 1.2 kb containing part of exon 5, intron 5, exon 6, and a small piece of intron 6 up to the EcoRV site.
Phenotypic informationDefects in glucose tolerance; defects on phagocytosis-associated crosstalk between macrophages and apoptotic cells; defective function of mitochondrial respiratory complex I.
Breeding historyBackcrossed for 10 generations.
References
  • Role of transglutaminase 2 in glucose tolerance: knockout mice studies and a putative mutation in a MODY patient.;Bernassola Francesca, Federici Massimo, Corazzari Marco, Terrinoni Alessandro, Hribal Marta L, De Laurenzi Vincenzo, Ranalli Marco, Massa Ornella, Sesti Giorgio, McLean W H Irwin, Citro Gennaro, Barbetti Fabrizio, Melino Gerry, ;2002;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;16;1371-8; 12205028
  • Transglutaminase 2-/- mice reveal a phagocytosis-associated crosstalk between macrophages and apoptotic cells.;Szondy Zsuzsa, Sarang Zsolt, Molnar Peter, Nemeth Tamas, Piacentini Mauro, Mastroberardino Pier Giorgio, Falasca Laura, Aeschlimann Daniel, Kovacs Judit, Kiss Ildiko, Szegezdi Eva, Lakos Gabriella, Rajnavolgyi Eva, Birckbichler Paul J, Melino Gerry, Fesus Laszlo, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;7812-7; 12810961
  • Transglutaminase 2 ablation leads to defective function of mitochondrial respiratory complex I affecting neuronal vulnerability in experimental models of extrapyramidal disorders.;Battaglia Giuseppe, Farrace Maria Grazia, Mastroberardino Pier Giorgio, Viti Irene, Fimia Gian Maria, Van Beeumen Jozef, Devreese Bart, Melino Gennaro, Molinaro Gemma, Busceti Carla Letizia, Biagioni Francesca, Nicoletti Ferdinando, Piacentini Mauro, ;2007;Journal of neurochemistry;100;36-49; 17064362

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI allele-associated human disease models

MGI phenotypes (allele matching)
  • altered response to myocardial infarction / MGI
  • abnormal cardiovascular system physiology / MGI
  • premature death / MGI
  • disorganized myocardium / MGI
  • increased myocardial infarction size / MGI
  • increased tumor growth/size / MGI
  • renal/urinary system phenotype / MGI
  • cardiovascular system phenotype / MGI
  • immune system phenotype / MGI
  • increased macrophage cell number / MGI
  • ventricular fibrillation / MGI
  • decreased susceptibility to endotoxin shock / MGI
  • decreased sensitivity to induced morbidity/mortality / MGI
  • abnormal mitochondrial ATP synthesis coupled electron transport / MGI
  • decreased hematocrit / MGI
  • hyperglycemia / MGI
  • decreased circulating insulin level / MGI
  • decreased erythrocyte cell number / MGI
  • increased insulin sensitivity / MGI
  • impaired glucose tolerance / MGI
  • increased physiological sensitivity to xenobiotic / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • abnormal thymus morphology / MGI
  • impaired macrophage phagocytosis / MGI
  • homeostasis/metabolism phenotype / MGI

Literature references

  • Role of transglutaminase 2 in glucose tolerance: knockout mice studies and a putative mutation in a MODY patient.;Bernassola Francesca, Federici Massimo, Corazzari Marco, Terrinoni Alessandro, Hribal Marta L, De Laurenzi Vincenzo, Ranalli Marco, Massa Ornella, Sesti Giorgio, McLean W H Irwin, Citro Gennaro, Barbetti Fabrizio, Melino Gerry, ;2002;FASEB journal : official publication of the Federation of American Societies for Experimental Biology;16;1371-8; 12205028
  • Transglutaminase 2-/- mice reveal a phagocytosis-associated crosstalk between macrophages and apoptotic cells.;Szondy Zsuzsa, Sarang Zsolt, Molnar Peter, Nemeth Tamas, Piacentini Mauro, Mastroberardino Pier Giorgio, Falasca Laura, Aeschlimann Daniel, Kovacs Judit, Kiss Ildiko, Szegezdi Eva, Lakos Gabriella, Rajnavolgyi Eva, Birckbichler Paul J, Melino Gerry, Fesus Laszlo, ;2003;Proceedings of the National Academy of Sciences of the United States of America;100;7812-7; 12810961
  • Transglutaminase 2 ablation leads to defective function of mitochondrial respiratory complex I affecting neuronal vulnerability in experimental models of extrapyramidal disorders.;Battaglia Giuseppe, Farrace Maria Grazia, Mastroberardino Pier Giorgio, Viti Irene, Fimia Gian Maria, Van Beeumen Jozef, Devreese Bart, Melino Gennaro, Molinaro Gemma, Busceti Carla Letizia, Biagioni Francesca, Nicoletti Ferdinando, Piacentini Mauro, ;2007;Journal of neurochemistry;100;36-49; 17064362

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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