B6;129-Gt(ROSA)26Sortm1.1(CAG-cre)Jphe/Orl
Status | Available to order |
EMMA ID | EM:02418 |
International strain name | B6;129-Gt(ROSA)26Sortm1.1(CAG-cre)Jphe/Orl |
Alternative name | DiCre |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Gt(ROSA)26Sortm1.1(CAG-cre)Jphe, |
Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
Provider | Jean-Paul Herman |
Provider affiliation | CNRS - Université de la Méditerranée |
Genetic information | The DiCre strain is a conditional deleter strain that is inducible by dimerization. It corresponds to two inactive moieties of Cre recombinase, Cre 19-59 and Cre 60-343, fused to FKBP12 and the ligand binding domain (FRB) of FRAP, respectively. The moieties have no recombinase activity, even when co-expressed in a cell. However, they can be dimerized by rapamycin, restoring recombinase activity. The construct which has been knocked into the ROSA 26 locus consists of: FRB-Cre(60-343)_CAG promoter_FKBP12-Cre(19-59). |
Phenotypic information | None observed. |
Breeding history | F1 animals were backcrossed with C57BL/6 animals for two generations, and inbred thereafter to obtain homozygous animals. They are presently inbred. |
References |
|
Information from EMMA
Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Literature references
- Regulation of Cre recombinase by ligand-induced complementation of inactive fragments.;Jullien Nicolas, Sampieri François, Enjalbert Alain, Herman Jean-Paul, ;2003;Nucleic acids research;31;e131; 14576331
- Conditional transgenesis using Dimerizable Cre (DiCre).;Jullien Nicolas, Goddard Isabelle, Selmi-Ruby Samia, Fina Jean-Luc, Cremer Harold, Herman Jean-Paul, ;2007;PloS one;2;e1355; 18159238
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).