CBy.Cg-Cd44tm1.1Ugu Tg(DO11.10)10Dlo/Cnrm

Status

Available to order

EMMA IDEM:02427
International strain nameCBy.Cg-Cd44tm1.1Ugu Tg(DO11.10)10Dlo/Cnrm
Alternative nameBALB/cByJIco DO11.10 CD44v6v7-/-
Strain typeTransgenic Strains
Allele/Transgene symbolTg(DO11.10)10Dlo,
Gene/Transgene symbolTg(DO11.10)10Dlo

Information from provider

ProviderUrsula Günthert
Provider affiliationInstitut für Pathologie, University of Basel
Genetic informationA loxP site was inserted 3' of exon v6 and a neomycin resistance cassette, with a 5' loxP site, was inserted 5' of exon v6 of the Cd44 gene. During insertion, 1.6 kb of sequence was lost in the exon v7 region; cre recombinase was transiently expressed in ES cells allowing the removal of neomycin cassette and exon v6. Mice lack both exon v6 and v7. The DO11.10 transgenic line was generated by oocyte injection of a cDNA encoding the T cell receptor DO11.10, reactive to the chicken ovalbumin peptide 323-339.
Phenotypic informationDO11.10: Expresses transgenic TCR that recognizes chicken ovalbumin in the context of I-Ad (class II restricted). Cd44v6v7-/-. Strongly reduced symptoms in autoimmune diseases and haemopoiesis.
References
  • Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
  • Induction by antigen of intrathymic apoptosis of CD4+CD8+TCRlo thymocytes in vivo.;Murphy K M, Heimberger A B, Loh D Y, ;1990;Science (New York, N.Y.);250;1720-3; 2125367

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • decreased T cell proliferation / MGI
  • abnormal digestive system physiology / MGI
  • immune system phenotype / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal T-helper 2 physiology / MGI

Literature references

  • Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
  • Induction by antigen of intrathymic apoptosis of CD4+CD8+TCRlo thymocytes in vivo.;Murphy K M, Heimberger A B, Loh D Y, ;1990;Science (New York, N.Y.);250;1720-3; 2125367

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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