STOCK Mgmt^tm1Iz Tg(CRP-cre)1Iz/Cnrm

Status	Available to order
EMMA ID	EM:00262
International strain name	STOCK Mgmt^tm1Iz Tg(CRP-cre)1Iz/Cnrm
Alternative name	floxed(mgmt)-Tg/hCRP-Cre
Strain type	Targeted Mutant Strains : Conditional mutation
Allele/Transgene symbol	Mgmt^tm1Iz,
Gene/Transgene symbol	Mgmt

Information from provider

Provider	Manfred Rajewsky
Provider affiliation	Institut fuer Zellbiologie (Tumorforschung), Universitaetsklinikum Essen
Genetic information	The cre recombinase gene was cloned under control of the human C-reactive protein (hCRP) promoter. The hCRP-promoter is inducible by lipopolysaccharide (LPS) or interleukin 6. Upon induction the cre gene is strongly expressed in hepatocytes, exclusively. The amount of cre-mediated deletion can be controlled by the amount of LPS injected. The percentage of deletion is in the range of 13 to 45%. Exon V of Mgmt was flanked by two loxP-sites. This exon can be spliced out by cre recombinase thereby inactivating the DNA repair activity of the MGMT protein.
Phenotypic information	No major defects observed. The Tg(CRP-cre) transgene allows the liver-specific, inducible expression of cre recombinase.
References	Conditional liver-specific "knock-out" of the murine genes encoding 06-Alkylguanine-DNA Alkyltransferase (AGT) and AP-Endonuclease (APE).;Schweer T, Rajewsky MF;1998;J Biomol Struct Dyn.;16;180-1 (Abstr.);

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Literature references

Conditional liver-specific "knock-out" of the murine genes encoding 06-Alkylguanine-DNA Alkyltransferase (AGT) and AP-Endonuclease (APE).;Schweer T, Rajewsky MF;1998;J Biomol Struct Dyn.;16;180-1 (Abstr.);

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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STOCK Mgmttm1Iz Tg(CRP-cre)1Iz/Cnrm