STOCK Vezttm1.1Smc/Vezttm1.2Smc/Orl

Status

Available to order

EMMA IDEM:04326
International strain nameSTOCK Vezttm1.1Smc/Vezttm1.2Smc/Orl
Alternative nameVezatin flox5/null (or bi-loxP/null vezt strain)
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolVezttm1.2Smc,
Gene/Transgene symbolVezt

Information from provider

ProviderMarie-Christine SIMMLER
Provider affiliationNeurobiology and development, CNRS
Genetic informationVezatin is a integral membrane protein associated to E-cadherin adherens junctions. Exon 5 encodes the transmembranous domain. A conditional vezatin allele lacking exon 5 was generated by using a cre/loxP strategy (original vezt floxed line, EM:01817). This is an heterozygous line modified as follows: one chromosome is carrying an exon-5-only-floxed (Vezt flox5) vezatin allele, i.e. deleted of the floxed pPgk-1-neo-pA cassette (by partial cre recombinase activity) and one chromosome is carrying a null (defloxed) vezatin allele (after deletion of the exon5+neo floxed segment using the pgk1-cre).
Phenotypic informationUbiquitous targeted disruption of vezatin results in embryonic lethality due to a failure of epithelialization of the trophectoderm (Hyenne et al., 2007). Hairs cells targeted disruption results in noise-induced hearing loss (Balhoul et al., 2009).
Breeding historyMore than N10 breeding on a mixed/randomized 129/SvPas x B6N. Breeding procedures of heterozygous Vezt Flox5/null x Vezt Flox5/null will only produce Vezt Flox5/null (66%) and Vezt Flox5/Flox5 (33%) mice, since Vezt null/null is an embryonic lethal condition.
References
  • Conditional knock-out reveals that zygotic vezatin-null mouse embryos die at implantation.;Hyenne Vincent, Souilhol Céline, Cohen-Tannoudji Michel, Cereghini Silvia, Petit Christine, Langa Francina, Maro Bernard, Simmler Marie-Christine, ;2007;Mechanisms of development;124;449-62; 17452094
  • Vezatin, an integral membrane protein of adherens junctions, is required for the sound resilience of cochlear hair cells.;Bahloul Amel, Simmler Marie-Christine, Michel Vincent, Leibovici Michel, Perfettini Isabelle, Roux Isabelle, Weil Dominique, Nouaille Sylvie, Zuo Jian, Zadro Cristina, Licastro Danilo, Gasparini Paolo, Avan Paul, Hardelin Jean-Pierre, Petit Christine, ;2009;EMBO molecular medicine;1;125-38; 20049712
  • Vezatin is essential for dendritic spine morphogenesis and functional synaptic maturation.;Danglot Lydia, Freret Thomas, Le Roux Nicolas, Narboux Nême Nicolas, Burgo Andrea, Hyenne Vincent, Roumier Anne, Contremoulins Vincent, Dauphin François, Bizot Jean-Charles, Vodjdani Guilan, Gaspar Patricia, Boulouard Michel, Poncer Jean-Christophe, Galli Thierry, Simmler Marie-Christine, ;2012;The Journal of neuroscience : the official journal of the Society for Neuroscience;32;9007-22; 22745500
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous 129/Sv x C57BL/6N, heterozygous 129/Sv x C57BL/6N

Disease and phenotype information

MGI phenotypes (allele matching)
  • failure of embryo implantation / MGI
  • abnormal cell adhesion / MGI
  • failure of blastocyst to hatch from the zona pellucida / MGI
  • delayed blastocyst hatching from the zona pellucida / MGI
  • embryonic lethality at implantation, complete penetrance / MGI

Literature references

  • Conditional knock-out reveals that zygotic vezatin-null mouse embryos die at implantation.;Hyenne Vincent, Souilhol Céline, Cohen-Tannoudji Michel, Cereghini Silvia, Petit Christine, Langa Francina, Maro Bernard, Simmler Marie-Christine, ;2007;Mechanisms of development;124;449-62; 17452094
  • Vezatin, an integral membrane protein of adherens junctions, is required for the sound resilience of cochlear hair cells.;Bahloul Amel, Simmler Marie-Christine, Michel Vincent, Leibovici Michel, Perfettini Isabelle, Roux Isabelle, Weil Dominique, Nouaille Sylvie, Zuo Jian, Zadro Cristina, Licastro Danilo, Gasparini Paolo, Avan Paul, Hardelin Jean-Pierre, Petit Christine, ;2009;EMBO molecular medicine;1;125-38; 20049712
  • Vezatin is essential for dendritic spine morphogenesis and functional synaptic maturation.;Danglot Lydia, Freret Thomas, Le Roux Nicolas, Narboux Nême Nicolas, Burgo Andrea, Hyenne Vincent, Roumier Anne, Contremoulins Vincent, Dauphin François, Bizot Jean-Charles, Vodjdani Guilan, Gaspar Patricia, Boulouard Michel, Poncer Jean-Christophe, Galli Thierry, Simmler Marie-Christine, ;2012;The Journal of neuroscience : the official journal of the Society for Neuroscience;32;9007-22; 22745500

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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