B6Rcc.Cg-Cd44tm1.1Ugu KitW/Cnrm

Status

Available to order

EMMA IDEM:04330
International strain nameB6Rcc.Cg-Cd44tm1.1Ugu KitW/Cnrm
Alternative nameC57 BL/6RCC c-kitW CD44v6v7-/-
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCd44tm1.1Ugu,
Gene/Transgene symbolCd44

Information from provider

ProviderUrsula Günthert
Provider affiliationInstitut für Pathologie, University of Basel
Genetic informationTargeting construct was derived from 129 DNA. Cd44tm1.1Ugu(Cd44v6v7): targeted deletion of both exons, leaving the rest of the gene intact. Alternative splicing of all variant exons is intact, except for the missing exons v6 and v7. c-KitW/W mice (Cd117-/-) carry a G to A nucleotide exchange in the W allele, which affects the splice donor site of intron 10. This causes skipping of exon 10 (encoding the transmembrane domain) and missplicing of exon 9 to exon 11. The W mutant of c-Kit (Cd117) lacks the transmembrane domain and cannot be expressed on the surface.
Phenotypic informationKitW(Cd117-/-): Macrocytic anaemia, reduced red blood cells number with increased volume. Cd44v6v7-/-: Strongly reduced symptoms in colitis, rheumatoid arthritis and experimental autoimmune encephalomyelitis, defects in haemopoiesis.
Breeding historyBackcrossed for 10 generations onto C57BL/6Rcc; then bred as C57BL/6Rcc Cd117 +/? Cd44v6v7?/?. Only the Cd44v6v7 mutation is kept homozygous, Cd117 mutation has to be kept heterozygous. Cd117-/- are not viable.
References
  • Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
  • Variant isoforms of CD44 are required in early thymocyte development.;Schwärzler C, Oliferenko S, Günthert U, ;2001;European journal of immunology;31;2997-3005; 11592076
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredyes
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • decreased T cell proliferation / MGI
  • abnormal digestive system physiology / MGI
  • immune system phenotype / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal T-helper 2 physiology / MGI
  • abnormal ventral coat pigmentation / MGI
  • decreased mast cell number / MGI
  • abnormal mast cell physiology / MGI
  • absent coat pigmentation / MGI
  • small ovary / MGI
  • small testis / MGI
  • abnormal spermatogenesis / MGI
  • infertility / MGI
  • abnormal ovarian follicle number / MGI
  • high mean erythrocyte cell number / MGI
  • increased hematocrit / MGI
  • irregular coat pigmentation / MGI
  • abnormal coat/hair pigmentation / MGI
  • variable body spotting / MGI
  • reproductive system phenotype / MGI
  • hematopoietic system phenotype / MGI
  • decreased body weight / MGI
  • prenatal lethality / MGI
  • macrocytic anemia / MGI
  • abnormal survival / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • abnormal eye pigmentation / MGI
  • postnatal lethality, complete penetrance / MGI
  • altered response to myocardial infarction / MGI
  • kidney inflammation / MGI
  • decreased inflammatory response / MGI
  • decreased airway responsiveness / MGI
  • increased urine protein level / MGI
  • increased myocardial infarction size / MGI
  • decreased vascular permeability / MGI
  • abnormal response/metabolism to endogenous compounds / MGI
  • abnormal renal glomerulus morphology / MGI
  • impaired neutrophil recruitment / MGI
  • abnormal interferon level / MGI
  • abnormal interleukin level / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • increased erythrocyte protoporphyrin level / MGI
  • glomerular crescent / MGI
  • abnormal Sertoli cell morphology / MGI
  • abnormal histamine physiology / MGI
  • decreased susceptibility to induced arthritis / MGI
  • abnormal body temperature / MGI
  • decreased susceptibility to type I hypersensitivity reaction / MGI
  • decreased basophil cell number / MGI
  • hepatic steatosis / MGI
  • preweaning lethality, incomplete penetrance / MGI
  • increased papilloma incidence / MGI
  • increased esophageal papilloma incidence / MGI
  • peptic ulcer / MGI
  • abnormal response to transplant / MGI
  • abnormal muscle physiology / MGI
  • abnormal interstitial cell of Cajal morphology / MGI
  • increased sensitivity to xenobiotic induced morbidity/mortality / MGI
  • prenatal lethality, complete penetrance / MGI

Literature references

  • Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
  • Variant isoforms of CD44 are required in early thymocyte development.;Schwärzler C, Oliferenko S, Günthert U, ;2001;European journal of immunology;31;2997-3005; 11592076

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
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For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

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