C;129P2-Thratm2Ven/Kctt

Status

Available to order

EMMA IDEM:04560
International strain nameC;129P2-Thratm2Ven/Kctt
Alternative nameThra-tm2Ven
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolThratm2Ven,
Gene/Transgene symbolThra

Information from provider

ProviderBjörn Vennström
Provider affiliationDepartment of Cell and Molecular Biology, Karolinska Institutet, CMB
Genetic informationThe RNAs for the thyroid hormone receptor alpha (Thra) TRa1 and TRa2 isoforms appear to be produced by a use of alternative transcriptional stops and subsequent splicing. Expression of the TRa2 isoform of the thyroid hormone receptor was completely abolished by abrogating transcription into exon 10, i.e. the exon that encodes the TRa2-specific coding sequence. As a result, all RNAs transcribed from the Thra locus by default encode the TRa1 isoform, and at elevated levels. Both RNA and protein levels for TRa1 are increased approx. 6-fold in the homozygote, 3-fold in the heterozygote. The artificial transcriptional stop is provided by the inserted neomycin-resistance cassette.
Phenotypic informationAnalysis of the phenotype revealed a mixed hypo- and hyperthyroid phenotype in both the homo- and heterozygotes. These features include moderately reduced levels of serum thyroid hormones, an increased heart rate, elevated body temperature and delayed ossification. These characteristics were ascribed to the elevated expression of TRa1, whereas no phenotype could be associated with the lack of TRa2 expression (Salto et al., 2001). The elevated expression of TRa1 can substitute for Thr-beta in Thrb-/- mice (Ng et al., 2001).
Breeding historyThe founders were backcrossed for approx 4 generations against BALB/c. The strain has been maintained as a heterozygote.
References
  • Ablation of TRalpha2 and a concomitant overexpression of alpha1 yields a mixed hypo- and hyperthyroid phenotype in mice.;Saltó C, Kindblom J M, Johansson C, Wang Z, Gullberg H, Nordström K, Mansén A, Ohlsson C, Thorén P, Forrest D, Vennström B, ;2001;Molecular endocrinology (Baltimore, Md.);15;2115-28; 11731613
  • A thyroid hormone receptor that is required for the development of green cone photoreceptors.;Ng L, Hurley J B, Dierks B, Srinivas M, Saltó C, Vennström B, Reh T A, Forrest D, ;2001;Nature genetics;27;94-8; 11138006
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

    • Resistance to thyroid hormone due to a mutation in thyroid hormone receptor alpha / Orphanet_566231
MGI phenotypes (allele matching)
  • decreased bone mineral density / MGI
  • abnormal trabecular bone morphology / MGI
  • obese / MGI
  • decreased body weight / MGI
  • postnatal growth retardation / MGI
  • increased heart rate / MGI
  • abnormal thyroid physiology / MGI
  • increased activity of thyroid / MGI
  • decreased activity of thyroid / MGI
  • abnormal compact bone morphology / MGI
  • abnormal thyroid-stimulating hormone level / MGI
  • decreased circulating insulin-like growth factor I level / MGI
  • decreased circulating thyroxine level / MGI
  • decreased circulating triiodothyronine level / MGI
  • increased body temperature / MGI
  • increased total body fat amount / MGI
  • decreased compact bone area / MGI
  • abnormal pup retrieval / MGI
  • abnormal osteoclast physiology / MGI
  • reduced female fertility / MGI
  • abnormal thyroid follicle morphology / MGI
  • prolonged estrous cycle / MGI
  • decreased bone mineral content / MGI
  • perinatal lethality, incomplete penetrance / MGI
  • decreased areal bone mineral density / MGI
  • hearing/vestibular/ear phenotype / MGI

Literature references

  • Ablation of TRalpha2 and a concomitant overexpression of alpha1 yields a mixed hypo- and hyperthyroid phenotype in mice.;Saltó C, Kindblom J M, Johansson C, Wang Z, Gullberg H, Nordström K, Mansén A, Ohlsson C, Thorén P, Forrest D, Vennström B, ;2001;Molecular endocrinology (Baltimore, Md.);15;2115-28; 11731613
  • A thyroid hormone receptor that is required for the development of green cone photoreceptors.;Ng L, Hurley J B, Dierks B, Srinivas M, Saltó C, Vennström B, Reh T A, Forrest D, ;2001;Nature genetics;27;94-8; 11138006

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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