FVB-Tg(tetO-TPR/MET,-EGFP)12Tcre/Cnrm

Status

Available to order

EMMA IDEM:04778
International strain nameFVB-Tg(tetO-TPR/MET,-EGFP)12Tcre/Cnrm
Alternative nameTpr-Met-TetO-GFP
Strain typeTransgenic Strains
Allele/Transgene symbolTg(tetO-TPR/MET, -EGFP)12Tcre
Gene/Transgene symbolTg(tetO-TPR/MET,-EGFP)12Tcre

Information from provider

ProviderTiziana Crepaldi
Provider affiliationAnatomy Pharmacology and Forensic Medicine, University of Turin
Additional ownerProf. Carola Ponzetto, Dept. Anatomy, Pharmacology and Forensic Medicine, Turin, Italy
Genetic informationIn order to obtain tetracycline controlled expression of human TRP-MET oncoprotein, its cDNA was cloned into the pBI-eGFP vector (Clontech) to generate the bidirectional Tpr-Met-TetO-GFP responder. Gene expression was induced in the heart by crossing the Tpr-Met-TetO-GFP transgenic line with mice which carry a transgene consisting of the tTA driven by cardiac alpha-MHC promoter. The GFP gene facilitates detection of responder transgene on whole organ as well as in tissue sections.
Phenotypic informationNo phenotype was observed in single transgenic mice.
References
  • Conditional activation of MET in differentiated skeletal muscle induces atrophy.;Crepaldi Tiziana, Bersani Francesca, Scuoppo Claudio, Accornero Paolo, Prunotto Chiara, Taulli Riccardo, Forni Paolo E, Leo Christian, Chiarle Roberto, Griffiths Jennifer, Glass David J, Ponzetto Carola, ;2007;The Journal of biological chemistry;282;6812-22; 17194700
  • A mouse model for spatial and temporal expression of HGF in the heart.;Riess Ilan, Sala Valentina, Leo Christian, Demaria Marco, Gatti Stefano, Gallo Simona, Fitou Amandine, Boero Ombretta, Levi Renzo, Cuccovillo Ivan, Molla Fabiola, De Angelis Noeleen, Staszewsky Lidia, Latini Roberto, Crepaldi Tiziana, ;2011;Transgenic research;20;1203-16; 21360304
  • A New Transgenic Mouse Model of Heart Failure and Cardiac Cachexia Raised by Sustained Activation of Met Tyrosine Kinase in the Heart.;Sala Valentina, Gatti Stefano, Gallo Simona, Medico Enzo, Cantarella Daniela, Cimino James, Ponzetto Antonio, Crepaldi Tiziana, ;2016;BioMed research international;2016;9549036; 27298830
  • Cardiac concentric hypertrophy promoted by activated Met receptor is mitigated in vivo by inhibition of Erk1,2 signalling with Pimasertib.;Sala Valentina, Gallo Simona, Gatti Stefano, Medico Enzo, Vigna Elisa, Cantarella Daniela, Fontani Lara, Natale Massimo, Cimino James, Morello Mara, Comoglio Paolo Maria, Ponzetto Antonio, Crepaldi Tiziana, ;2016;Journal of molecular and cellular cardiology;93;84-97; 26924269
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Literature references

  • Conditional activation of MET in differentiated skeletal muscle induces atrophy.;Crepaldi Tiziana, Bersani Francesca, Scuoppo Claudio, Accornero Paolo, Prunotto Chiara, Taulli Riccardo, Forni Paolo E, Leo Christian, Chiarle Roberto, Griffiths Jennifer, Glass David J, Ponzetto Carola, ;2007;The Journal of biological chemistry;282;6812-22; 17194700
  • A mouse model for spatial and temporal expression of HGF in the heart.;Riess Ilan, Sala Valentina, Leo Christian, Demaria Marco, Gatti Stefano, Gallo Simona, Fitou Amandine, Boero Ombretta, Levi Renzo, Cuccovillo Ivan, Molla Fabiola, De Angelis Noeleen, Staszewsky Lidia, Latini Roberto, Crepaldi Tiziana, ;2011;Transgenic research;20;1203-16; 21360304
  • A New Transgenic Mouse Model of Heart Failure and Cardiac Cachexia Raised by Sustained Activation of Met Tyrosine Kinase in the Heart.;Sala Valentina, Gatti Stefano, Gallo Simona, Medico Enzo, Cantarella Daniela, Cimino James, Ponzetto Antonio, Crepaldi Tiziana, ;2016;BioMed research international;2016;9549036; 27298830
  • Cardiac concentric hypertrophy promoted by activated Met receptor is mitigated in vivo by inhibition of Erk1,2 signalling with Pimasertib.;Sala Valentina, Gallo Simona, Gatti Stefano, Medico Enzo, Vigna Elisa, Cantarella Daniela, Fontani Lara, Natale Massimo, Cimino James, Morello Mara, Comoglio Paolo Maria, Ponzetto Antonio, Crepaldi Tiziana, ;2016;Journal of molecular and cellular cardiology;93;84-97; 26924269

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
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Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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