B6.Cg-Tg(ITGAM-cre)2781Gkl/Flmg

Status	Available to order
EMMA ID	EM:04788
International strain name	B6.Cg-Tg(ITGAM-cre)2781Gkl/Flmg
Alternative name	CD11b-Cre
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(ITGAM-cre)2781Gkl,
Gene/Transgene symbol	Tg(ITGAM-cre)2781Gkl

Information from provider

Provider	George Kollias
Provider affiliation	B.S.R.C.
Genetic information	For the generation of CD11b-Cre transgenic mice, a 1.7kb DNA fragment (position -1584kb - +85bp) of the human ITGAM (CD11b) promoter (Hickstein et al., 1992) was cloned by amplification, from genomic DNA derived from the human leukemic myelomonocytic cell line U937. Sense primer: 5'-CGGGGTACCGGTTCAAAGTGATTCTGC-3' introduced a KpnI restriction site, antisense primer: 5'-CCGCTCGAGTGGAAGGAGCCAGAACC-3' created a XhoI restriction site at position + 85bp of the promoter. The KpnI-XhoI fragment was cloned to respective restriction sites of the pBluescript (Stratagene) vector containing the cre recombinase gene. As a polyadenylation signal, the human growth hormone (2.2Kb) gene was used. The transgene was isolated for microinjection into fertilized eggs as a KpnI-NotI fragment.
Phenotypic information	No abnormal phenotype detected. This strain expresses cre recombinase under the human ITGAM (CD11b) promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, cre mediated recombination results in deletion of the targeted sequence specifically in peritoneal macrophages and microglial cells. This strain represents an effective tool for the generation of cell specific targeted mutants.
References	Onset and progression in inherited ALS determined by motor neurons and microglia.;Boillée Séverine, Yamanaka Koji, Lobsiger Christian S, Copeland Neal G, Jenkins Nancy A, Kassiotis George, Kollias George, Cleveland Don W, ;2006;Science (New York, N.Y.);312;1389-92; 16741123

Information from EMMA

Archiving centre	B.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archiving	heterozygous C57BL/6J, wild-type C57BL/6J
Stage of embryos	Morula

Literature references

Onset and progression in inherited ALS determined by motor neurons and microglia.;Boillée Séverine, Yamanaka Koji, Lobsiger Christian S, Copeland Neal G, Jenkins Nancy A, Kassiotis George, Kollias George, Cleveland Don W, ;2006;Science (New York, N.Y.);312;1389-92; 16741123

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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