B6.129S-Tnftm3Gkl/Flmg

Status

Available to order

EMMA IDEM:04791
International strain nameB6.129S-Tnftm3Gkl/Flmg
Alternative nameTnf-tm/tm
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolTnftm3Gkl,
Gene/Transgene symbolTnf

Information from provider

ProviderGeorge Kollias
Provider affiliationB.S.R.C.
Genetic informationThe genomic locus containing the genes for Tnf and lymphotoxin a, was isolated from a 129Sv mouse genomic library in lamdaFIXTMII. The replacement-type targeting vector was made by replacing the 724 bp BglII-SmaI fragment containing the second and third exons of Tnf with a mutated 511 bp BglII-SmaI fragment carrying a deletion of the first twelve amino acids of soluble Tnf (Tnf delta1-12). A pL2neo gene flanked by loxP sites was inserted into the first intron of the Tnf gene at the BglII site, and a PGK tk gene was inserted at the 5' end of the targeting vector for negative selection. The targeting vector contained 4.9 kb homologous DNA upstream of the first loxP site and 0.7 kb homologous DNA downstream of the Tnf delta1-12 deletion. The targeting vector was electroporated into CCE ES cells.
Phenotypic informationMutant mice produce physiologically regulated levels of transmembrane TNF (tmTNF), but cannot substitute for the hepatotoxic activities of wild-type TNF following LPS/D-galactosamine challenge in vivo and are not sufficient to support secondary lymphoid organ structure and function.
References
  • Transmembrane TNF protects mutant mice against intracellular bacterial infections, chronic inflammation and autoimmunity.;Alexopoulou Lena, Kranidioti Ksanthi, Xanthoulea Sofia, Denis Maria, Kotanidou Anastasia, Douni Eleni, Blackshear Perry J, Kontoyiannis Dimitris L, Kollias George, ;2006;European journal of immunology;36;2768-80; 16983719

Information from EMMA

Archiving centreB.S.R.C. Alexander Fleming, Vari, Greece

Disease and phenotype information

MGI phenotypes (allele matching)
  • autoimmune response / MGI
  • abnormal lymph node B cell domain morphology / MGI
  • decreased susceptibility to bacterial infection / MGI
  • abnormal lymphocyte physiology / MGI
  • decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
  • abnormal mesenteric lymph node morphology / MGI
  • absent follicular dendritic cells / MGI
  • abnormal spleen primary B follicle morphology / MGI
  • absent spleen germinal center / MGI
  • decreased IgG1 level / MGI
  • decreased circulating tumor necrosis factor level / MGI
  • decreased tumor necrosis factor secretion / MGI
  • decreased susceptibility to endotoxin shock / MGI

Literature references

  • Transmembrane TNF protects mutant mice against intracellular bacterial infections, chronic inflammation and autoimmunity.;Alexopoulou Lena, Kranidioti Ksanthi, Xanthoulea Sofia, Denis Maria, Kotanidou Anastasia, Douni Eleni, Blackshear Perry J, Kontoyiannis Dimitris L, Kollias George, ;2006;European journal of immunology;36;2768-80; 16983719

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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