- autoimmune response / MGI
- abnormal lymph node B cell domain morphology / MGI
- decreased susceptibility to bacterial infection / MGI
- abnormal lymphocyte physiology / MGI
- decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
- abnormal mesenteric lymph node morphology / MGI
- absent follicular dendritic cells / MGI
- abnormal spleen primary B follicle morphology / MGI
- absent spleen germinal center / MGI
- decreased IgG1 level / MGI
- decreased circulating tumor necrosis factor level / MGI
- decreased tumor necrosis factor secretion / MGI
- decreased susceptibility to endotoxin shock / MGI
B6.129S-Tnftm3Gkl/Flmg
Status | Available to order |
EMMA ID | EM:04791 |
International strain name | B6.129S-Tnftm3Gkl/Flmg |
Alternative name | Tnf-tm/tm |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Tnftm3Gkl, |
Gene/Transgene symbol | Tnf |
Information from provider
Provider | George Kollias |
Provider affiliation | B.S.R.C. |
Genetic information | The genomic locus containing the genes for Tnf and lymphotoxin a, was isolated from a 129Sv mouse genomic library in lamdaFIXTMII. The replacement-type targeting vector was made by replacing the 724 bp BglII-SmaI fragment containing the second and third exons of Tnf with a mutated 511 bp BglII-SmaI fragment carrying a deletion of the first twelve amino acids of soluble Tnf (Tnf delta1-12). A pL2neo gene flanked by loxP sites was inserted into the first intron of the Tnf gene at the BglII site, and a PGK tk gene was inserted at the 5' end of the targeting vector for negative selection. The targeting vector contained 4.9 kb homologous DNA upstream of the first loxP site and 0.7 kb homologous DNA downstream of the Tnf delta1-12 deletion. The targeting vector was electroporated into CCE ES cells. |
Phenotypic information | Mutant mice produce physiologically regulated levels of transmembrane TNF (tmTNF), but cannot substitute for the hepatotoxic activities of wild-type TNF following LPS/D-galactosamine challenge in vivo and are not sufficient to support secondary lymphoid organ structure and function. |
References |
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Information from EMMA
Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
Disease and phenotype information
MGI phenotypes (allele matching)
Literature references
- Transmembrane TNF protects mutant mice against intracellular bacterial infections, chronic inflammation and autoimmunity.;Alexopoulou Lena, Kranidioti Ksanthi, Xanthoulea Sofia, Denis Maria, Kotanidou Anastasia, Douni Eleni, Blackshear Perry J, Kontoyiannis Dimitris L, Kollias George, ;2006;European journal of immunology;36;2768-80; 16983719
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