C57BL/6N-Gt(ROSA)26Sor^{tm1(FLP1)Wtsi}/WtsiIeg

Status	Available to order
EMMA ID	EM:04830
International strain name	C57BL/6N-Gt(ROSA)26Sor^{tm1(FLP1)Wtsi}/WtsiIeg
Alternative name	ROSA26 Fki
Strain type	Targeted Mutant Strains : Knock-in
Allele/Transgene symbol	Gt(ROSA)26Sor^{tm1(FLP1)Wtsi},
Gene/Transgene symbol	Gt(ROSA)26Sor

Information from provider

Provider	Haydn Prosser
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	The mouse strain is a repetition of the targeted knock-in of the FLPeR construct targeted into the Gt(ROSA)26Sor locus using C57BL/6N ES cells (JM8-F6). Original reference for the targeting construct: Widespread recombinase expression using FLPeR (flipper) mice. Farley FW, Soriano P, Steffen LS, Dymecki SM. Genesis. 2000 Nov-Dec;28(3-4):106-10. Reference for the ES cells: Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR, Lloyd KC, Bradley A, Skarnes WC. Agouti C57BL/6N embryonic stem cells for mouse genetic resources. Nat Methods. 2009 Jul;6(7):493-5. Epub 2009 Jun 14.
Phenotypic information	This is a germline deleter strain intended to delete sequence flanked by two Flp recombinase recognition (FRT) sites.
Breeding history	The allele was maintained as an inbred line in the C57BL/6N (Taconic) background.
References	Widespread recombinase expression using FLPeR (flipper) mice.;Farley F W, Soriano P, Steffen L S, Dymecki S M, ;2000;Genesis (New York, N.Y. : 2000);28;106-10; 11105051 Agouti C57BL/6N embryonic stem cells for mouse genetic resources.;Pettitt Stephen J, Liang Qi, Rairdan Xin Y, Moran Jennifer L, Prosser Haydn M, Beier David R, Lloyd Kent C, Bradley Allan, Skarnes William C, ;2009;Nature methods;6;493-5; 19525957
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany

Literature references

Widespread recombinase expression using FLPeR (flipper) mice.;Farley F W, Soriano P, Steffen L S, Dymecki S M, ;2000;Genesis (New York, N.Y. : 2000);28;106-10; 11105051
Agouti C57BL/6N embryonic stem cells for mouse genetic resources.;Pettitt Stephen J, Liang Qi, Rairdan Xin Y, Moran Jennifer L, Prosser Haydn M, Beier David R, Lloyd Kent C, Bradley Allan, Skarnes William C, ;2009;Nature methods;6;493-5; 19525957

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.

C57BL/6N-Gt(ROSA)26Sortm1(FLP1)Wtsi/WtsiIeg