B6.129S2-Cdkn1atm1Tyj/RvoCnbc

Status

Available to order

EMMA IDEM:04869
International strain nameB6.129S2-Cdkn1atm1Tyj/RvoCnbc
Alternative nameB6-Cdkn1atm1Tyj/JRvO
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCdkn1atm1Tyj,
Gene/Transgene symbolCdkn1a

Information from provider

ProviderRonald van Os
Provider affiliationMolecular Genetics, University Medical Center Groningen
Genetic informationB6;129S2-Cdkn1atm1Tyj/J were backcrossed for 10 generations on a C57BL/6 background resulting in a new strain: B6-Cdkn1atm1Tyj/JRvO.
Phenotypic informationIn contrast to the same mutation on a 129 background, the C57BL/6 background has no phenotype in hematopoietic stem cells. Overall, the mice have a higher sensitivity to ionizing radiation.
Breeding historyB6;129S2-Cdkn1atm1Tyj/J were backcrossed for 10 generations on a C57BL/6 background resulting in a new strain: B6-Cdkn1atm1Tyj/JRvO. Currently, mice are bred as one homozygous male with one homozygous female.
References
  • A Limited role for p21Cip1/Waf1 in maintaining normal hematopoietic stem cell functioning.;van Os Ronald, Kamminga Leonie M, Ausema Albertina, Bystrykh Leonid V, Draijer Deanna P, van Pelt Kyrjon, Dontje Bert, de Haan Gerald, ;2007;Stem cells (Dayton, Ohio);25;836-43; 17170062
  • Radiation-induced cell cycle arrest compromised by p21 deficiency.;Brugarolas J, Chandrasekaran C, Gordon J I, Beach D, Jacks T, Hannon G J, ;1995;Nature;377;552-7; 7566157
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivinghomozygous C57BL/6, homozygous C57BL/6
Stage of embryos8-cell

Disease and phenotype information

MGI phenotypes (allele matching)
  • increased malignant tumor incidence / MGI
  • increased incidence of tumors by ionizing radiation induction / MGI
  • decreased monocyte cell number / MGI
  • abnormal bone marrow cell morphology/development / MGI
  • decreased susceptibility to induced arthritis / MGI
  • impaired macrophage chemotaxis / MGI
  • hearing/vestibular/ear phenotype / MGI
  • cardiovascular system phenotype / MGI
  • increased brown adipose tissue amount / MGI
  • increased cell proliferation / MGI
  • increased body weight / MGI
  • decreased body weight / MGI
  • increased brain weight / MGI
  • altered response to CNS ischemic injury / MGI
  • increased kidney weight / MGI
  • increased hematopoietic stem cell number / MGI
  • decreased seminal vesicle weight / MGI
  • abnormal neuronal precursor proliferation / MGI
  • decreased prostate gland weight / MGI
  • increased neuronal precursor cell number / MGI
  • increased circulating cholesterol level / MGI
  • abnormal DNA replication / MGI
  • increased cellular sensitivity to ultraviolet irradiation / MGI
  • decreased physiological sensitivity to xenobiotic / MGI
  • increased sensitivity to induced cell death / MGI
  • increased neuron number / MGI
  • increased white fat cell number / MGI
  • increased inguinal fat pad weight / MGI
  • increased parametrial fat pad weight / MGI
  • abnormal prostate gland dorsolateral lobe morphology / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • increased sensitivity to xenobiotic induced morbidity/mortality / MGI
  • abnormal hematopoietic stem cell physiology / MGI
  • abnormal junctional epithelium morphology / MGI
  • abnormal keratinocyte physiology / MGI
  • increased squamous cell carcinoma incidence / MGI
  • abnormal skin physiology / MGI
  • abnormal cell physiology / MGI
  • delayed cellular replicative senescence / MGI
  • enlarged spleen / MGI
  • spleen hyperplasia / MGI
  • enlarged lymph nodes / MGI
  • decreased sensitivity to skin irradiation / MGI
  • increased cellular sensitivity to gamma-irradiation / MGI
  • increased tumor incidence / MGI
  • increased B cell derived lymphoma incidence / MGI
  • increased T cell derived lymphoma incidence / MGI
  • increased carcinoma incidence / MGI
  • premature death / MGI
  • abnormal CD4-positive, alpha beta T cell morphology / MGI
  • glomerulonephritis / MGI
  • increased urine protein level / MGI
  • decreased mortality induced by ionizing radiation / MGI
  • kidney failure / MGI
  • increased hemangiosarcoma incidence / MGI
  • abnormal thymus physiology / MGI
  • increased incidence of tumors by chemical induction / MGI
  • decreased incidence of tumors by ionizing radiation induction / MGI
  • increased anti-double stranded DNA antibody level / MGI
  • increased anti-nuclear antigen antibody level / MGI
  • increased anti-histone antibody level / MGI
  • increased spleen weight / MGI
  • abnormal self tolerance / MGI
  • increased B cell number / MGI
  • increased T cell proliferation / MGI
  • immune system phenotype / MGI
  • increased memory T cell number / MGI
  • lymph node hyperplasia / MGI
  • increased IgG1 level / MGI
  • enlarged cervical lymph nodes / MGI
  • increased lung carcinoma incidence / MGI
  • increased histiocytic sarcoma incidence / MGI
  • increased Leydig cell tumor incidence / MGI
  • increased urinary system tumor incidence / MGI
  • increased skin tumor incidence / MGI
  • increased sebaceous gland adenoma incidence / MGI
  • increased metastatic potential / MGI

Literature references

  • A Limited role for p21Cip1/Waf1 in maintaining normal hematopoietic stem cell functioning.;van Os Ronald, Kamminga Leonie M, Ausema Albertina, Bystrykh Leonid V, Draijer Deanna P, van Pelt Kyrjon, Dontje Bert, de Haan Gerald, ;2007;Stem cells (Dayton, Ohio);25;836-43; 17170062
  • Radiation-induced cell cycle arrest compromised by p21 deficiency.;Brugarolas J, Chandrasekaran C, Gordon J I, Beach D, Jacks T, Hannon G J, ;1995;Nature;377;552-7; 7566157

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).