B6.129(SJL)-Slc6a3tm1(cre)Xz/Cnrm

Status

Available to order

EMMA IDEM:04893
International strain nameB6.129(SJL)-Slc6a3tm1(cre)Xz/Cnrm
Alternative nameDAT-cre
Strain typeTargeted Mutant Strains : Knock-in
Allele/Transgene symbolSlc6a3tm1(cre)Xz,
Gene/Transgene symbolSlc6a3

Information from provider

ProviderRene Hen
Provider affiliationColumbia University
Genetic informationA targeting vector was constructed by inserting a cassette with the cre recombinase coding sequence, a nuclear translocation signal, and a PGK-neo gene flanked by FRT sites, into the 5'-UTR of the Slc6a3 (Dat) gene. After homologous recombination in ES cells, mice with cre recombinase expressed from the Slc6a3 gene were produced. Knock-in mice were crossed with transgenic flp-deleter mice to remove the neo marker. A line was established where the neo was removed and the flp recombinase transgene was absent. Cre recombinase expression is detected in the ventral tegmental area (VAT) and substantia nigra zona compacta (SNc).
Phenotypic informationNo abnormal phenotype detected.
Breeding historyThe line was backcrossed in C57BL/6 for 10 generations and it is currently maintained by siblings breedings.
References
  • Targeted gene expression in dopamine and serotonin neurons of the mouse brain.;Zhuang Xiaoxi, Masson Justine, Gingrich Jay A, Rayport Stephen, Hen René, ;2005;Journal of neuroscience methods;143;27-32; 15763133
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • hyperactivity / MGI

Literature references

  • Targeted gene expression in dopamine and serotonin neurons of the mouse brain.;Zhuang Xiaoxi, Masson Justine, Gingrich Jay A, Rayport Stephen, Hen René, ;2005;Journal of neuroscience methods;143;27-32; 15763133

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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