B6;129P2-Cftrtm1Unc/Orl

Status

Available to order

EMMA IDEM:00051
International strain nameB6;129P2-Cftrtm1Unc/Orl
Alternative nameCftr°
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCftrtm1Unc,
Gene/Transgene symbolCftr

Information from provider

ProviderBeverly H. Koller
Provider affiliationc/o CDTA-Orleans
Phenotypic informationMutant mice deficient in Cftr gene. Obtained by homologous recombination at exon 10 of the Cftr gene (insertion of the neomycin-resistance gene). No modification of the phenotype among the heterozygous population. Homozygotes show anomalies similar to human mucoviscidosis found in young patients. Most of these animals die of intestinal obstruction before mature age.
Breeding history129/Ola ES cells; 3 generations of backcross to C57BL/6, then intercross. Breeding heterozygous x heterozygous.
References
  • An animal model for cystic fibrosis made by gene targeting.;Snouwaert J N, Brigman K K, Latour A M, Malouf N N, Boucher R C, Smithies O, Koller B H, ;1992;Science (New York, N.Y.);257;1083-8; 1380723

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous 129/Sv x C57BL/6, wild-type C57BL/6
Stage of embryos2-cell

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • impaired fertilization / MGI
  • reduced male fertility / MGI
  • decreased litter size / MGI
  • asthenozoospermia / MGI
  • impaired sperm capacitation / MGI
  • transmission ratio distortion / MGI
  • small ovary / MGI
  • abnormal uterine cervix morphology / MGI
  • decreased body weight / MGI
  • postnatal growth retardation / MGI
  • lung inflammation / MGI
  • reduced female fertility / MGI
  • delayed sexual maturation / MGI
  • small uterus / MGI
  • decreased corpora lutea number / MGI
  • intestinal obstruction / MGI
  • decreased ovulation rate / MGI
  • abnormal sperm physiology / MGI
  • abnormal phospholipid level / MGI
  • decreased ovary weight / MGI
  • decreased uterus weight / MGI
  • homeostasis/metabolism phenotype / MGI
  • prolonged estrous cycle / MGI
  • absent estrous cycle / MGI
  • thin uterus / MGI
  • pancreatic acinar cell zymogen granule accumulation / MGI
  • ileum hypertrophy / MGI
  • increased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • postnatal lethality, incomplete penetrance / MGI
  • decreased respiratory epithelial chloride transmembrane transport / MGI
  • decreased respiratory epithelial sodium ion transmembrane transport / MGI
  • decreased intestinal epithelial sodium ion transmembrane transport / MGI
  • pancreatic acinus dilation / MGI
  • abnormal intestine morphology / MGI
  • abnormal bronchus morphology / MGI
  • abnormal pulmonary alveolus morphology / MGI
  • pulmonary interstitial fibrosis / MGI
  • increased lung compliance / MGI
  • abnormal crypts of Lieberkuhn morphology / MGI
  • abnormal colon morphology / MGI
  • spleen hyperplasia / MGI
  • decreased body size / MGI
  • distended abdomen / MGI
  • abnormal gait / MGI
  • abnormal thymus involution / MGI
  • female infertility / MGI
  • abnormal pancreas morphology / MGI
  • premature death / MGI
  • abnormal gland morphology / MGI
  • abnormal nasal mucosa morphology / MGI
  • abnormal paranasal sinus morphology / MGI
  • abnormal trachea morphology / MGI
  • peritoneal inflammation / MGI
  • abnormal submandibular gland morphology / MGI
  • small pancreas / MGI
  • abnormal gallbladder morphology / MGI
  • dilated gallbladder / MGI
  • coiled cecum / MGI
  • increased respiratory mucosa goblet cell number / MGI
  • abnormal Brunner's gland morphology / MGI
  • abnormal trachea gland morphology / MGI
  • abnormal intestinal goblet cell morphology / MGI
  • decreased intestinal epithelial chloride transmembrane transport / MGI
  • dilated crypts of Lieberkuhn / MGI
  • abnormal vital capacity / MGI
  • respiratory system phenotype / MGI

Literature references

  • An animal model for cystic fibrosis made by gene targeting.;Snouwaert J N, Brigman K K, Latour A M, Malouf N N, Boucher R C, Smithies O, Koller B H, ;1992;Science (New York, N.Y.);257;1083-8; 1380723

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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