B6;129-Polktm1.1Rsky Polhtm1Crey PoliGt(S15-7G1)Sor/Orl

Status

Available to order

EMMA IDEM:05122
International strain nameB6;129-Polktm1.1Rsky Polhtm1Crey PoliGt(S15-7G1)Sor/Orl
Alternative nameDNA polymerases etaXiotaXkappa KO
Strain typeGene-trap
Allele/Transgene symbolPoliGt(S15-7G1)Sor,
Gene/Transgene symbolPoli

Information from provider

ProviderClaude-Agnes Reynaud
Provider affiliationFaculte de Medecine Paris Descartes - Site Broussais, Unite INSERM 783 "Developpement du systeme immunitaire"
Genetic informationPol eta: the gene-targeting construct results in the deletion of exon 4, which contains the DE polymerase motifs conserved in all Y-family polymerases and indispensable for their catalytic function. Pol iota: the gene-trap vector (Rosafary) was introduced between exons 1 and 2 in the ES cells. Pol kappa: the gene-targeting construct results in the deletion of exon 6, which contains two essential catalytic residues.
Phenotypic informationIncreased incidence of UV-induced skin cancer. Decreased immunoglobulin somatic hypermutation at A/T base pairs.
Breeding historyFor each strain the heterozygotes were obtained by breeding chimeras with C57BL/6 mice. These strains were crossed together to obtain triple knock-out mice.
References
  • Contribution of DNA polymerase eta to immunoglobulin gene hypermutation in the mouse.;Delbos Frédéric, De Smet Annie, Faili Ahmad, Aoufouchi Said, Weill Jean-Claude, Reynaud Claude-Agnès, ;2005;The Journal of experimental medicine;201;1191-6; 15824086
  • Role of DNA polymerases eta, iota and zeta in UV resistance and UV-induced mutagenesis in a human cell line.;Gueranger Quentin, Stary Anne, Aoufouchi Saïd, Faili Ahmad, Sarasin Alain, Reynaud Claude-Agnès, Weill Jean-Claude, ;2008;DNA repair;7;1551-62; 18586118
  • A backup role of DNA polymerase kappa in Ig gene hypermutation only takes place in the complete absence of DNA polymerase eta.;Faili Ahmad, Stary Anne, Delbos Frédéric, Weller Sandra, Aoufouchi Said, Sarasin Alain, Weill Jean-Claude, Reynaud Claude-Agnès, ;2009;Journal of immunology (Baltimore, Md. : 1950);182;6353-9; 19414788
  • 129-Derived Mouse Strains Express an Unstable but Catalytically Active DNA Polymerase Iota Variant.;Aoufouchi Said, De Smet Annie, Delbos Frédéric, Gelot Camille, Guerrera Ida Chiara, Weill Jean-Claude, Reynaud Claude-Agnès, ;2015;Molecular and cellular biology;35;3059-70; 26124279
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivinghomozygous C57BL/6J, homozygous C57BL/6J

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • abnormal B cell physiology / MGI
  • immune system phenotype / MGI
  • abnormal DNA repair / MGI
  • increased cellular sensitivity to ultraviolet irradiation / MGI

Literature references

  • Contribution of DNA polymerase eta to immunoglobulin gene hypermutation in the mouse.;Delbos Frédéric, De Smet Annie, Faili Ahmad, Aoufouchi Said, Weill Jean-Claude, Reynaud Claude-Agnès, ;2005;The Journal of experimental medicine;201;1191-6; 15824086
  • Role of DNA polymerases eta, iota and zeta in UV resistance and UV-induced mutagenesis in a human cell line.;Gueranger Quentin, Stary Anne, Aoufouchi Saïd, Faili Ahmad, Sarasin Alain, Reynaud Claude-Agnès, Weill Jean-Claude, ;2008;DNA repair;7;1551-62; 18586118
  • A backup role of DNA polymerase kappa in Ig gene hypermutation only takes place in the complete absence of DNA polymerase eta.;Faili Ahmad, Stary Anne, Delbos Frédéric, Weller Sandra, Aoufouchi Said, Sarasin Alain, Weill Jean-Claude, Reynaud Claude-Agnès, ;2009;Journal of immunology (Baltimore, Md. : 1950);182;6353-9; 19414788
  • 129-Derived Mouse Strains Express an Unstable but Catalytically Active DNA Polymerase Iota Variant.;Aoufouchi Said, De Smet Annie, Delbos Frédéric, Gelot Camille, Guerrera Ida Chiara, Weill Jean-Claude, Reynaud Claude-Agnès, ;2015;Molecular and cellular biology;35;3059-70; 26124279

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