C57BL/6J-Lim2Aca47/Ieg

Status

Available to order

EMMA IDEM:05135
International strain nameC57BL/6J-Lim2Aca47/Ieg
Alternative nameAca47
Strain typeInduced Mutant Strains : Chemically-induced
Allele/Transgene symbolLim2Aca47,
Gene/Transgene symbolLim2

Information from provider

ProviderOliver Puk
Provider affiliationInstitut fuer Entwicklungsgenetik, AG Graw, Helmholtz Zentrum Muenchen
Genetic informationENU induced Lim2 C51R missense mutation.

Disclaimer - Special restrictions:

1) Mutant development was phenotype driven. Although for some mutant lines, linkage data and the molecular characterization of the causative mutation has been published, EMMA has not genotyped the strains and can not provide genotyping protocols.
2) Some lines showed low penetrance of the phenotype.
3) Only sperm available. Rederivation service can not be offered.
4) EMMA quality control standards may not apply for these strains.
5) EMMA has not verified the breeding performance and the genetic background of the strains.

The genotype/phenotype of the mice is not confirmed by INFRAFRONTIER/EMMA. We strongly recommend that the recipient confirms genotype/phenotype upon receipt.
Phenotypic informationAca47 is characterized by microphthalmia and microphakia with congenital cataracts.
Breeding historyIncross, 10 generations.
References
  • Microphakia and congenital cataract formation in a novel Lim2(C51R) mutant mouse.;Puk Oliver, Ahmad Nafees, Wagner Sibylle, de Angelis Martin Hrabé, Graw Jochen, ;2011;Molecular vision;17;1164-71; 21617753
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivinghomozygous C57BL/6J, wild-type C57BL/6J
Stage of embryos2-cell

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • abnormal lens morphology / MGI
  • small lens / MGI
  • abnormal eye size / MGI
  • vision/eye phenotype / MGI
  • cataract / MGI
  • abnormal lens fiber morphology / MGI
  • nuclear cataracts / MGI

Literature references

  • Microphakia and congenital cataract formation in a novel Lim2(C51R) mutant mouse.;Puk Oliver, Ahmad Nafees, Wagner Sibylle, de Angelis Martin Hrabé, Graw Jochen, ;2011;Molecular vision;17;1164-71; 21617753

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).