B6Dnk;B6Brd;B6N-Tyrc-Brd Sparctm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:05296
International strain nameB6Dnk;B6Brd;B6N-Tyrc-Brd Sparctm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0060_1_A07
Strain typeTargeted Mutant Strains
Allele/Transgene symbolSparctm1a(EUCOMM)Wtsi,
Gene/Transgene symbolSparc
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0060_1_A07. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
ReferencesNone available

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • decreased bone mineral density / IMPC
  • abnormal tibia morphology / IMPC
  • decreased body length / IMPC
  • decreased body weight / IMPC
  • abnormal lens morphology / IMPC
  • cataract / IMPC
  • abnormal coat/hair pigmentation / IMPC
  • abnormal eye morphology / IMPC
  • abnormal tooth morphology / IMPC
  • increased heart weight / IMPC
  • decreased heart weight / IMPC
  • increased circulating alanine transaminase level / IMPC
  • abnormal humerus morphology / IMPC
  • abnormal incisor color / IMPC
  • decreased bone mineral content / IMPC
MGI phenotypes (allele matching)
  • abnormal tibia morphology / MGI
  • decreased body weight / MGI
  • abnormal lens morphology / MGI
  • cataract / MGI
  • abnormal coat/hair pigmentation / MGI
  • abnormal eye morphology / MGI
  • abnormal tooth morphology / MGI
  • abnormal humerus morphology / MGI
  • decreased mean corpuscular hemoglobin concentration / MGI
  • abnormal incisor color / MGI

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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