FVB/N-Tg(Rnu6-RNAi:IE-PRV)SH05Gjo/Orl
| Status | Available to order |
| EMMA ID | EM:05365 |
| International strain name | FVB/N-Tg(Rnu6-RNAi:IE-PRV)SH05Gjo/Orl |
| Alternative name | SH05 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Rnu6-RNAi:IE-PRV)SH05Gjo, |
| Gene/Transgene symbol | Tg(Rnu6-RNAi:IE-PRV)SH05Gjo |
Information from provider
| Provider | Genevieve Jolivet |
| Provider affiliation | INRA, INRA |
| Genetic information | Transgenic mice for a U6-shRNA gene. 4 copies of the transgene are integrated. The U6-shRNA construct encompasses the U6 mouse promoter that drives the transcription of a shRNA expressing transcription unit. The shRNA sequence targets the 3'-part of the intermediate early gene (IE gene) of the Pseudorabies virus (PRV), with the following sequence (5'-CTCCCATCGGGTGAGAAAA-3'). PRV currently infects farm animals (especially pigs) and mice. The U6-shRNA gene is inserted in an empty transgene expression vector encompassing the 5'HS4 insulator from the chicken beta globin gene locus, the human eF1alpha gene promoter (-1430, +2), the rabbit (rb) beta-globin second intron and several enhancers (E1, E2 and E3). The empty expression vector acts as a cargo for the U6-shRNA gene to insure the reliable expression. |
| Phenotypic information | Stable production of siRNA targeting the IE RNA. Resistance to a PRV infection (Bartha strain). No stimulation of the innate immune system in non infected animals. |
| Breeding history | Currently bred with wild-type mice. Stable over 5 generations. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous FVB/N, wild-type FVB/N |
Literature references
- Viral infection resistance conferred on mice by siRNA transgenesis.;Daniel-Carlier Nathalie, Sawafta Ashraf, Passet Bruno, Thépot Dominique, Leroux-Coyau Mathieu, Lefèvre François, Houdebine Louis-Marie, Jolivet Geneviève, ;2013;Transgenic research;22;489-500; 22961198