CBA.129S(B6)-Tectatm1Gpr/H

Status

Available to order

EMMA IDEM:05539
International strain nameCBA.129S(B6)-Tectatm1Gpr/H
Alternative nameCBA/Tectatm1Gpr
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolTectatm1Gpr,
Gene/Transgene symbolTecta

Information from provider

ProviderGuy Richardson
Provider affiliationAncillary Unit, University of Sussex
Genetic informationA neomycin resistance cassette was inserted into exon 3 of Tecta using a targeting construct derived from 129/SvEvBrd DNA.
Phenotypic informationHomozygous mutant mice suffer hearing loss and have detached tectorial membranes lacking all non-collagenous matrix. Insertion of the neo cassette results in exon skipping, producing a Tecta transcript in which exons 2 and 4 are joined. While the encoded protein is undetectable in homozygous mutant mice by Western blotting, immunofluorescence analysis shows immunoreactivity in the greater epithelial ridge and in a few small punctate spots in the tectorial membrane. Many mouse strains do not behave well under anaesthesia and/or suffer from age related hearing loss, especially in the high-frequency basal end of the cochlea. Neither the C57BL/6 nor the CBA strains mice are very suitable for recording intra- and extracellular potentials from cochlear hair cells under anaesthesia, and the C57BL/6 strain suffers from age related hearing loss (due to a mutation in the Cdh23 gene at the Ahl locus). Therefore, if the scientists who work with these mice want to do physiology on anaesthetised animals, it is proposed that they order both strains and use (CBA x C57BL/6)F1 hybrids for experimentation.
Breeding historyOriginal 129/SvEv:C57BL/6J mice have been outbred to CBA to F28.
References
  • A targeted deletion in alpha-tectorin reveals that the tectorial membrane is required for the gain and timing of cochlear feedback.;Legan P K, Lukashkina V A, Goodyear R J, Kössi M, Russell I J, Richardson G P, ;2000;Neuron;28;273-85; 11087000
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous CBA
Breeding at archiving centreMales were archived upon arrival. No breeding was performed at the archiving centre.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

    • Autosomal recessive non-syndromic sensorineural deafness type DFNB / Orphanet_90636
    • Autosomal dominant non-syndromic sensorineural deafness type DFNA / Orphanet_90635
MGI phenotypes (allele matching)
  • abnormal hearing physiology / MGI
  • abnormal otolithic membrane morphology / MGI
  • enlarged otoliths / MGI
  • decreased otolith number / MGI
  • abnormal tectorial membrane morphology / MGI
  • detached tectorial membrane / MGI
  • abnormal cochlear microphonics / MGI
  • decreased cochlear nerve compound action potential / MGI
  • abnormal hair cell mechanoelectric transduction / MGI
  • abnormal cochlear outer hair cell physiology / MGI
  • abnormal distortion product otoacoustic emission / MGI
  • absent distortion product otoacoustic emissions / MGI
  • behavior/neurological phenotype / MGI
  • abnormal tectorial membrane striated-sheet matrix morphology / MGI

Literature references

  • A targeted deletion in alpha-tectorin reveals that the tectorial membrane is required for the gain and timing of cochlear feedback.;Legan P K, Lukashkina V A, Goodyear R J, Kössi M, Russell I J, Richardson G P, ;2000;Neuron;28;273-85; 11087000

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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