CBA.129S(B6)-Tectbtm1Gpr/H

Status

Available to order

EMMA IDEM:05541
International strain nameCBA.129S(B6)-Tectbtm1Gpr/H
Alternative nameCBA/Tectbtm1Gpr
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolTectbtm1Gpr,
Gene/Transgene symbolTectb

Information from provider

ProviderGuy Richardson
Provider affiliationAncillary Unit, University of Sussex
Genetic informationExons 1 to 4 of the Tectb gene were deleted and replaced by a neomycin resistance cassette using a targeting construct derived from 129/SvEvBrd DNA.
Phenotypic informationHomozygous mutant mice suffer low frequency hearing loss and have normally attached tectorial membranes that lack striated sheet matrix. Hensen's stripe is missing and the marginal band is absent in the apical coil of the cochlea. Sharpness of high frequency tuning is enhanced. Many mouse strains do not behave well under anaesthesia and/or suffer from age related hearing loss, especially in the high-frequency basal end of the cochlea. Neither the C57BL/6 nor the CBA strains mice are very suitable for recording intra- and extracellular potentials from cochlear hair cells under anaesthesia, and the C57BL/6 strain suffers from age related hearing loss (due to a mutation in the Cdh23 gene at the Ahl locus). Therefore, if the scientists who work with these mice want to do physiology on anaesthetised animals, it is proposed that they order both strains and use (CBA x C57BL/6)F1 hybrids for experimentation.
Breeding historyOriginal 129/SvEv:C57BL/6J mice have been outbred to CBA to F23.
References
  • Sharpened cochlear tuning in a mouse with a genetically modified tectorial membrane.;Russell Ian J, Legan P Kevin, Lukashkina Victoria A, Lukashkin Andrei N, Goodyear Richard J, Richardson Guy P, ;2007;Nature neuroscience;10;215-23; 17220887
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous CBA
Breeding at archiving centreMales were archived upon arrival. No breeding was performed at the archiving centre.

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal tectorial membrane morphology / MGI
  • decreased cochlear nerve compound action potential / MGI
  • enlarged tectorial membrane / MGI
  • abnormal distortion product otoacoustic emission / MGI
  • impaired hearing / MGI
  • enhanced cochlear frequency tuning / MGI
  • absent Hensen stripe / MGI
  • abnormal tectorial membrane marginal band morphology / MGI
  • abnormal tectorial membrane striated-sheet matrix morphology / MGI

Literature references

  • Sharpened cochlear tuning in a mouse with a genetically modified tectorial membrane.;Russell Ian J, Legan P Kevin, Lukashkina Victoria A, Lukashkin Andrei N, Goodyear Richard J, Richardson Guy P, ;2007;Nature neuroscience;10;215-23; 17220887

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
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Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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