CBA.129S(B6)-Otoatm1Gpr/H

Status

Available to order

EMMA IDEM:05546
International strain nameCBA.129S(B6)-Otoatm1Gpr/H
Alternative nameCBA/OtoaEGFP
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolOtoatm1Gpr,
Gene/Transgene symbolOtoa

Information from provider

ProviderGuy Richardson
Provider affiliationAncillary Unit, University of Sussex
Genetic informationAn Otoa null mutant allele was generated by inserting EGFP in frame into exon 2 of Otoa using a targeting construct prepared from a 129/Sv BAC clone. EGFP is expressed under the control of the Otoa promoter.
Phenotypic informationIn null mutant (OtoaEGFP/EGFP) mice the tectorial membrane is detached at the spiral limbus but remains attached to the outer hair cells, Hensen's stripe is missing and the marginal band appears abnormal. Null mutants show hearing loss but excitation of the outer hair cells and cochlear amplification are almost normal. Many mouse strains do not behave well under anaesthesia and/or suffer from age related hearing loss, especially in the high-frequency basal end of the cochlea. Neither the C57BL/6 nor the CBA strains mice are very suitable for recording intra- and extracellular potentials from cochlear hair cells under anaesthesia, and the C57BL/6 strain suffers from age related hearing loss (due to a mutation in the Cdh23 gene at the Ahl locus). Therefore, if the scientists who work with these mice want to do physiology on anaesthetised animals, it is proposed that they order both strains and use (CBA x C57BL/6)F1 hybrids for experimentation.
Breeding historyOriginal 129/SvEv:C57BL/6J mice have been outbred to CBA to F13.
ReferencesNone available
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archivingheterozygous CBA
Breeding at archiving centreMales were archived upon arrival. No breeding was performed at the archiving centre.

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

    • Autosomal recessive non-syndromic sensorineural deafness type DFNB / Orphanet_90636
MGI phenotypes (allele matching)
  • abnormal tectorial membrane morphology / MGI
  • detached tectorial membrane / MGI
  • abnormal cochlear nerve compound action potential / MGI
  • hearing/vestibular/ear phenotype / MGI
  • impaired hearing / MGI
  • absent Hensen stripe / MGI

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
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Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

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