STOCK Apctm2.1Cip/Orl
| Status | Available to order |
| EMMA ID | EM:05566 |
| International strain name | STOCK Apctm2.1Cip/Orl |
| Alternative name | Apc-lox-exon14 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Apctm2.1Cip, |
| Gene/Transgene symbol | Apc |
Information from provider
| Provider | Sabine Colnot |
| Provider affiliation | INSERM |
| Additional owner | Dr M. Giovannini, Center for Neural Tumor Research, House Ear Institute, Los Angeles, CA, USA |
| Genetic information | Targeting construct is derived from 129 DNA. The loxP sites are located in the 13rd and in the 14th intron, and there is no selection cassette remaining in the floxed allele; cre-mediated recombination between the loxP sites will excise the 14th exon (Apc-delta14 allele) generating a stop codon at AA579. |
| Phenotypic information | After cre-mediated recombination, Apc protein loses its domain required for beta-catenin degradation. Consequently, it activates a sustained beta-catenin signaling. |
| Breeding history | 4 backcrosses to C57BL/6 background and cryopreserved after the first homozygous mating. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J, wild-type C57BL/6J |
Disease and phenotype information
MGI allele-associated human disease models
Orphanet associated rare diseases, based on orthologous gene matching
- Desmoid tumor / Orphanet_873
- Cenani-Lenz syndrome / Orphanet_3258
- Turcot syndrome with polyposis / Orphanet_99818
- Gastric adenocarcinoma and proximal polyposis of the stomach / Orphanet_314022
- Gardner syndrome / Orphanet_79665
- APC-related attenuated familial adenomatous polyposis / Orphanet_247806
Literature references
- Cre-mediated germline mosaicism: a new transgenic mouse for the selective removal of residual markers from tri-lox conditional alleles.;Leneuve Patricia, Colnot Sabine, Hamard Ghislaine, Francis Fiona, Niwa-Kawakita Michiko, Giovannini Marco, Holzenberger Martin, ;2003;Nucleic acids research;31;e21; 12595570
- Colorectal cancers in a new mouse model of familial adenomatous polyposis: influence of genetic and environmental modifiers.;Colnot Sabine, Niwa-Kawakita Michiko, Hamard Ghislaine, Godard Cécile, Le Plenier Servane, Houbron Christophe, Romagnolo Béatrice, Berrebi Dominique, Giovannini Marco, Perret Christine, ;2004;Laboratory investigation; a journal of technical methods and pathology;84;1619-30; 15502862
- Liver-targeted disruption of Apc in mice activates beta-catenin signaling and leads to hepatocellular carcinomas.;Colnot S, Decaens T, Niwa-Kawakita M, Godard C, Hamard G, Kahn A, Giovannini M, Perret C, ;2004;Proceedings of the National Academy of Sciences of the United States of America;101;17216-21; 15563600
- Crypt-restricted proliferation and commitment to the Paneth cell lineage following Apc loss in the mouse intestine.;Andreu Pauline, Colnot Sabine, Godard Cécile, Gad Sophie, Chafey Philippe, Niwa-Kawakita Michiko, Laurent-Puig Pierre, Kahn Axel, Robine Sylvie, Perret Christine, Romagnolo Béatrice, ;2005;Development (Cambridge, England);132;1443-51; 15716339
- Apc tumor suppressor gene is the "zonation-keeper" of mouse liver.;Benhamouche Samira, Decaens Thomas, Godard Cécile, Chambrey Régine, Rickman David S, Moinard Christophe, Vasseur-Cognet Mireille, Kuo Calvin J, Kahn Axel, Perret Christine, Colnot Sabine, ;2006;Developmental cell;10;759-70; 16740478
- Stabilization of beta-catenin affects mouse embryonic liver growth and hepatoblast fate.;Decaens Thomas, Godard Cécile, de Reyniès Aurélien, Rickman David S, Tronche François, Couty Jean-Pierre, Perret Christine, Colnot Sabine, ;2008;Hepatology (Baltimore, Md.);47;247-58; 18038450