B6.129X1-Spo11tm1Mjn/Orl

Status

Available to order

EMMA IDEM:05730
International strain nameB6.129X1-Spo11tm1Mjn/Orl
Alternative nameB.Cg-Spo11tm1Sky H2aw9/Orl ou Spo11_C57BL/6J
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolSpo11tm1Mjn,
Gene/Transgene symbolSpo11

Information from provider

ProviderFrederic BAUDAT
Provider affiliationUPR1142 CNRS, Institut de Génétique Humaine
Genetic informationExons 4 to 6 of Spo11 were replaced with a neomycin resistance cassette, removing the putative catalytic tyrosine encoded by exon 5. The Spo11 gene has been isolated from a 129/SvJ mouse genomic DNA library. A double-stranded linker containing the I-SceI recognition sequence was inserted at the NotI site of the vector pPNT, leading to the duplication of the NotI site. Then, a 2.1 kb HindIII/XbaI genomic fragment containing Spo11 exons 2 and 3 was inserted at the XbaI site. Finally, a 7.5 kb HindIII genomic fragment with exons 7-13 was inserted at the XhoI site of this intermediate, yielding the targeting vector pLSPR.
Phenotypic informationHomozygotes for the targeted null mutation are sterile. Mutant males exhibit loss of spermatocytes in early prophase, while mutant females exhibit oocyte loss soon after birth.
Breeding historyKnock-out construct introduced in 129X1 ES cells. Backcrossed with C57BL/6J for 11 generations. After that current inbreeding.
References
  • Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11.;Baudat F, Manova K, Yuen J P, Jasin M, Keeney S, ;2000;Molecular cell;6;989-98; 11106739
  • Recombinational DNA double-strand breaks in mice precede synapsis.;Mahadevaiah S K, Turner J M, Baudat F, Rogakou E P, de Boer P, Blanco-Rodríguez J, Jasin M, Keeney S, Bonner W M, Burgoyne P S, ;2001;Nature genetics;27;271-6; 11242108
  • Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants.;Di Giacomo Monica, Barchi Marco, Baudat Frédéric, Edelmann Winfried, Keeney Scott, Jasin Maria, ;2005;Proceedings of the National Academy of Sciences of the United States of America;102;737-42; 15640358
  • Surveillance of different recombination defects in mouse spermatocytes yields distinct responses despite elimination at an identical developmental stage.;Barchi Marco, Mahadevaiah Shantha, Di Giacomo Monica, Baudat Frédéric, de Rooij Dirk G, Burgoyne Paul S, Jasin Maria, Keeney Scott, ;2005;Molecular and cellular biology;25;7203-15; 16055729
Homozygous fertileno
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous C57BL/6J, wild-type C57BL/6J

Disease and phenotype information

MGI phenotypes (allele matching)
  • small ovary / MGI
  • abnormal meiosis / MGI
  • decreased ovary weight / MGI
  • abnormal female meiosis / MGI
  • decreased oocyte number / MGI
  • abnormal synaptonemal complex / MGI
  • abnormal male meiosis / MGI
  • arrest of male meiosis / MGI
  • abnormal chromosomal synapsis / MGI
  • abnormal double-strand DNA break repair / MGI
  • abnormal ovary morphology / MGI
  • impaired ovarian folliculogenesis / MGI
  • small seminiferous tubules / MGI
  • arrest of spermatogenesis / MGI
  • abnormal spermatogenesis / MGI
  • male infertility / MGI
  • female infertility / MGI
  • abnormal oogenesis / MGI
  • decreased mature ovarian follicle number / MGI
  • decreased testis weight / MGI
  • azoospermia / MGI
  • immune system phenotype / MGI
  • abnormal spermatid morphology / MGI
  • abnormal primordial ovarian follicle morphology / MGI
  • oocyte degeneration / MGI

Literature references

  • Chromosome synapsis defects and sexually dimorphic meiotic progression in mice lacking Spo11.;Baudat F, Manova K, Yuen J P, Jasin M, Keeney S, ;2000;Molecular cell;6;989-98; 11106739
  • Recombinational DNA double-strand breaks in mice precede synapsis.;Mahadevaiah S K, Turner J M, Baudat F, Rogakou E P, de Boer P, Blanco-Rodríguez J, Jasin M, Keeney S, Bonner W M, Burgoyne P S, ;2001;Nature genetics;27;271-6; 11242108
  • Distinct DNA-damage-dependent and -independent responses drive the loss of oocytes in recombination-defective mouse mutants.;Di Giacomo Monica, Barchi Marco, Baudat Frédéric, Edelmann Winfried, Keeney Scott, Jasin Maria, ;2005;Proceedings of the National Academy of Sciences of the United States of America;102;737-42; 15640358
  • Surveillance of different recombination defects in mouse spermatocytes yields distinct responses despite elimination at an identical developmental stage.;Barchi Marco, Mahadevaiah Shantha, Di Giacomo Monica, Baudat Frédéric, de Rooij Dirk G, Burgoyne Paul S, Jasin Maria, Keeney Scott, ;2005;Molecular and cellular biology;25;7203-15; 16055729

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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