129S2(Cg)-Dcxtm1.2Ffr/Orl

Status

Available to order

EMMA IDEM:05808
International strain name129S2(Cg)-Dcxtm1.2Ffr/Orl
Alternative nameDcx knockout Sv129Pas
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolDcxtm1.2Ffr,
Gene/Transgene symbolDcx

Information from provider

ProviderFiona Francis
Provider affiliationInserm UMRS 839
Genetic informationTo generate Dcx knock-out mice (Kappeler et al, 2006) a loxP site containing a BamHI restriction site was inserted in the BsaI site upstream of Dcx exon 3. A floxed PGK-neo selection gene was inserted into the XbaI site downstream of exon 3. Mice carrying a floxed Dcx allele, with loxP sites flanking exon 3 and a floxed selection gene, were crossed with cre transgenic mice expressing the cre recombinase early in development (Leneuve et al, 2003). This cross generated (1) knock-out mice (having deleted Dcx exon 3 and the selection gene) and (2) mice deleted for the selection gene but still carrying the floxed exon 3 which will further allow the inactivation of Dcx in a spatially and temporally controlled manner. Mice are maintained on pure C57BL/6N and 129/SvPas backgrounds. We would like to cryopreserve knock-out mice on the 129/SvPas background. The Dcx gene is on the X chromosome and hence knock-out males are hemizygotes. Crosses with wild-type 129/SvPas females will produce heterozygous females and wild-type males.
Phenotypic informationThese constitutive knockout mice have hippocampus lamination defects and are a model of hippocampus dysplasia associated with epilepsy.
Breeding historyBackcrossing has been performed for at least 10 generations. For cryoconservation we would use hemizygous males crossed with wild-type females.
References
  • Branching and nucleokinesis defects in migrating interneurons derived from doublecortin knockout mice.;Kappeler Caroline, Saillour Yoann, Baudoin Jean-Pierre, Tuy Françoise Phan Dinh, Alvarez Chantal, Houbron Christophe, Gaspar Patricia, Hamard Ghislaine, Chelly Jamel, Métin Christine, Francis Fiona, ;2006;Human molecular genetics;15;1387-400; 16571605
  • Magnetic resonance imaging and histological studies of corpus callosal and hippocampal abnormalities linked to doublecortin deficiency.;Kappeler Caroline, Dhenain Marc, Phan Dinh Tuy Françoise, Saillour Yoann, Marty Serge, Fallet-Bianco Catherine, Souville Isabelle, Souil Evelyne, Pinard Jean-Marc, Meyer Gundela, Encha-Razavi Ferechté, Volk Andreas, Beldjord Cherif, Chelly Jamel, Francis Fiona, ;2007;The Journal of comparative neurology;500;239-54; 17111359
  • Epilepsy in Dcx knockout mice associated with discrete lamination defects and enhanced excitability in the hippocampus.;Nosten-Bertrand Marika, Kappeler Caroline, Dinocourt Céline, Denis Cécile, Germain Johanne, Phan Dinh Tuy Françoise, Verstraeten Soraya, Alvarez Chantal, Métin Christine, Chelly Jamel, Giros Bruno, Miles Richard, Depaulis Antoine, Francis Fiona, ;2008;PloS one;3;e2473; 18575605
  • Cellular anatomy, physiology and epileptiform activity in the CA3 region of Dcx knockout mice: a neuronal lamination defect and its consequences.;Bazelot Michael, Simonnet Jean, Dinocourt Céline, Bruel-Jungerman Elodie, Miles Richard, Fricker Desdemona, Francis Fiona, ;2012;The European journal of neuroscience;35;244-56; 22250815
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Animals used for archivingheterozygous 129S2/SvPas
Breeding at archiving centreHemizygous males with wild-type females
Stage of embryos2-cell

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

Literature references

  • Branching and nucleokinesis defects in migrating interneurons derived from doublecortin knockout mice.;Kappeler Caroline, Saillour Yoann, Baudoin Jean-Pierre, Tuy Françoise Phan Dinh, Alvarez Chantal, Houbron Christophe, Gaspar Patricia, Hamard Ghislaine, Chelly Jamel, Métin Christine, Francis Fiona, ;2006;Human molecular genetics;15;1387-400; 16571605
  • Magnetic resonance imaging and histological studies of corpus callosal and hippocampal abnormalities linked to doublecortin deficiency.;Kappeler Caroline, Dhenain Marc, Phan Dinh Tuy Françoise, Saillour Yoann, Marty Serge, Fallet-Bianco Catherine, Souville Isabelle, Souil Evelyne, Pinard Jean-Marc, Meyer Gundela, Encha-Razavi Ferechté, Volk Andreas, Beldjord Cherif, Chelly Jamel, Francis Fiona, ;2007;The Journal of comparative neurology;500;239-54; 17111359
  • Epilepsy in Dcx knockout mice associated with discrete lamination defects and enhanced excitability in the hippocampus.;Nosten-Bertrand Marika, Kappeler Caroline, Dinocourt Céline, Denis Cécile, Germain Johanne, Phan Dinh Tuy Françoise, Verstraeten Soraya, Alvarez Chantal, Métin Christine, Chelly Jamel, Giros Bruno, Miles Richard, Depaulis Antoine, Francis Fiona, ;2008;PloS one;3;e2473; 18575605
  • Cellular anatomy, physiology and epileptiform activity in the CA3 region of Dcx knockout mice: a neuronal lamination defect and its consequences.;Bazelot Michael, Simonnet Jean, Dinocourt Céline, Bruel-Jungerman Elodie, Miles Richard, Fricker Desdemona, Francis Fiona, ;2012;The European journal of neuroscience;35;244-56; 22250815

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

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