129S2.CD1-Tg(Myh6-Htr2b)1Lum/Orl
| Status | Available to order |
| EMMA ID | EM:05938 |
| International strain name | 129S2.CD1-Tg(Myh6-Htr2b)1Lum/Orl |
| Alternative name | MHC-5-HT2B in 129S2 |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Myh6-Htr2b)1Lum |
| Gene/Transgene symbol | Tg(Myh6-Htr2b)1Lum |
Information from provider
| Provider | Luc Maroteaux |
| Provider affiliation | Institut du Fer a Moulin, INSERM UMR S-839 |
| Genetic information | A 5.5 Kbp BamHI-KpnI restriction fragment of the mouse alpha-myosin heavy chain (MHC) was fused to a 2.0 Kbp XbaI-XbaI restriction fragment of mouse Htr2b (5-HT2BR) cDNA. The recombinant plasmid p-MHC-5-HT2B was linearized, microinjected into pronuclei of fertilized CD1 mouse eggs and implanted into pseudopregnant CD1 foster mothers. |
| Phenotypic information | The MHC-5-HT2B receptor transgenic mice exhibit hypertrophic heart with increased ventricular mass, abnormal cellular organization and proliferation of mitochondria. This was not accompanied with cardiovascular functional alterations. Seven-fold overexpression of the 5-HT2B receptor in the heart leads to compensated hypertrophic cardiomyopathy associated with proliferation of the mitochondria. This observation suggests a role for mitochondria in the hypertrophic signaling that is regulated by serotonin. |
| Breeding history | The original CD1 transgenic line was backcrossed more than 10 generations over the 129S2 strain and it is currently maintained over this 129S2 background. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous 129S2/SvPas, wild-type 129S2/SvPas |
| Breeding at archiving centre | Some wild-type females provided by the company potentially have a mixed background: B6.CBAx129/SvPas. It is possible that some frozen embryos have the same background. |
| Stage of embryos | 8-cell |
Disease and phenotype information
MGI allele-associated human disease models
Literature references
- Overexpression of the serotonin 5-HT2B receptor in heart leads to abnormal mitochondrial function and cardiac hypertrophy.;Nebigil Canan G, Jaffré Fabrice, Messaddeq Nadia, Hickel Pierre, Monassier Laurent, Launay Jean-Marie, Maroteaux Luc, ;2003;Circulation;107;3223-9; 12810613