STOCK Tg(CAMK2A-KCNIP3*,-lacZ)1Cnbc/Cnbc

Status	Available to order
EMMA ID	EM:06004
International strain name	STOCK Tg(CAMK2A-KCNIP3*,-lacZ)1Cnbc/Cnbc
Alternative name	B6CBA-Tg(hCaMKIIalpha-hEFmDREAM-IRES-lacZ)JN1Cnbc
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(CAMK2A-KCNIP3*,-lacZ)1Cnbc
Gene/Transgene symbol	Tg(CAMK2A-KCNIP3*,-lacZ)1Cnbc

Information from provider

Provider	Jose Ramon Naranjo
Provider affiliation	Centro Nacional de Biotecnologia (CNB-CSIC)
Genetic information	The cDNA encoding human KCNIP3 (DREAM) with two amino-acid substitutions at EF-hands 2, 3 and 4 (EFmDREAM) was inserted downstream of the human CaMKIIalpha promoter in a bicistronic expression vector containing an IRES and the lacZ reporter gene. The transgene cassette was microinjected into the pronuclei of one-cell embryos (B6CBAF2) and maintained as hemizygotes in mixed B6CBA background.
Phenotypic information	High transgene expression. Brain, immune system and reproductive system, expression detected throughout the entire spinal cord. The presence of lacZ reporter gene cassette within these transgenes makes them prone to display alterations in the expected gene expression pattern and levels.
Breeding history	Maintained in mixed B6CBA background.
References	Downstream regulatory element antagonist modulator regulates Ca2+ homeostasis and viability in cerebellar neurons.;Gomez-Villafuertes Rosa, Torres Begoña, Barrio Jorge, Savignac Magali, Gabellini Nadia, Rizzato Federico, Pintado Belen, Gutierrez-Adan Alfonso, Mellström Britt, Carafoli Ernesto, Naranjo Jose R, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;10822-30; 16306395 DREAM regulates BDNF-dependent spinal sensitization.;Rivera-Arconada Ivan, Benedet Tomaso, Roza Carolina, Torres Begoña, Barrio Jorge, Krzyzanowska Agnieszka, Avendaño Carlos, Mellström Britt, Lopez-Garcia José A, Naranjo José R, ;2010;Molecular pain;6;95; 21167062
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous (C57BL/6 x DBA/2)F2

Literature references

Downstream regulatory element antagonist modulator regulates Ca2+ homeostasis and viability in cerebellar neurons.;Gomez-Villafuertes Rosa, Torres Begoña, Barrio Jorge, Savignac Magali, Gabellini Nadia, Rizzato Federico, Pintado Belen, Gutierrez-Adan Alfonso, Mellström Britt, Carafoli Ernesto, Naranjo Jose R, ;2005;The Journal of neuroscience : the official journal of the Society for Neuroscience;25;10822-30; 16306395
DREAM regulates BDNF-dependent spinal sensitization.;Rivera-Arconada Ivan, Benedet Tomaso, Roza Carolina, Torres Begoña, Barrio Jorge, Krzyzanowska Agnieszka, Avendaño Carlos, Mellström Britt, Lopez-Garcia José A, Naranjo José R, ;2010;Molecular pain;6;95; 21167062

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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