B6.Cg-Tg(CAMK2A-KCNIP3*,-lacZ)33Cnbc/Cnbc

Status	Available to order
EMMA ID	EM:06007
International strain name	B6.Cg-Tg(CAMK2A-KCNIP3*,-lacZ)33Cnbc/Cnbc
Alternative name	B6.B6CBA-Tg(hCaMKIIalpha-hEFmDREAM-IRES-lacZ)DRE33Cnbc
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(CAMK2A-KCNIP3*,-lacZ)33Cnbc
Gene/Transgene symbol	Tg(CAMK2A-KCNIP3*,-lacZ)33Cnbc

Information from provider

Provider	Jose Ramon Naranjo
Provider affiliation	Centro Nacional de Biotecnologia (CNB-CSIC)
Genetic information	The cDNA encoding human KCNIP3 (DREAM) with two amino-acid substitutions at EF-hands 2, 3 and 4 (EFmDREAM) was inserted downstream of the human CaMK.IIalpha promoter in a bicistronic expression vector containing an IRES and the lacZ reporter gene. The transgene cassette was microinjected into the pronuclei of one-cell embryos (B6CBA) and backcrossed and maintained as hemizygotes in C57BL/6 background.
Phenotypic information	Moderate to low transgene expression. Brain, immune system and reproductive system, expression not detected in the entire spinal cord. The presence of lacZ reporter gene cassette within these transgenes makes them prone to display alterations in the expected gene expression pattern and levels.
Breeding history	Generated in B6CBA background and backcrossed (>10 times) to C57BL/6. Currently maintained as hemizygous in C57BL/6 background.
References	DREAM (downstream regulatory element antagonist modulator) contributes to synaptic depression and contextual fear memory.;Wu Long-Jun, Mellström Britt, Wang Hansen, Ren Ming, Domingo Sofia, Kim Susan S, Li Xiang-Yao, Chen Tao, Naranjo Jose R, Zhuo Min, ;2010;Molecular brain;3;3; 20205763
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archiving	heterozygous (C57BL/6 x CBA)F2
Stage of embryos	8-cell

Literature references

DREAM (downstream regulatory element antagonist modulator) contributes to synaptic depression and contextual fear memory.;Wu Long-Jun, Mellström Britt, Wang Hansen, Ren Ming, Domingo Sofia, Kim Susan S, Li Xiang-Yao, Chen Tao, Naranjo Jose R, Zhuo Min, ;2010;Molecular brain;3;3; 20205763

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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