B6.Cg-Tg(Gfap-tTA)7Igta Tg(tetO-Ppp3ca*)5Igta/Cnbc

Status

Available to order

EMMA IDEM:06201
International strain nameB6.Cg-Tg(Gfap-tTA)7Igta Tg(tetO-Ppp3ca*)5Igta/Cnbc
Alternative nameAIC (astrocyte inducible calcineurin)
Strain typeTransgenic Strains
Allele/Transgene symbolTg(tetO-Ppp3ca*)5Igta,
Gene/Transgene symbolTg(tetO-Ppp3ca*)5Igta

Information from provider

ProviderAna M. Fernandez
Provider affiliationFunctional and Systems Neurobiology, Instituto Cajal, CSIC
Genetic informationRegulated expression of a truncated, constitutively active form of calcineurin (CnA or Ppp3ca) in astrocytes was achieved with the Tet-off system by generating two transgenic mouse lines. A first mouse line expressed tet-transactivator (tTA) under the astrocyte-specific GFAP promoter. A second transgenic line expressed CnA under the control of the TetO promoter. A cDNA encoding a truncated form of the murine calcineurin catalytic subunit A, CnA (OKeefe et al., 1992), was placed downstream from the TetO promoter (from pTRE vector; Clontech). The pGFAPtTA and TetOCnA DNA fragments were purified and independently injected into fertilized eggs of C57BL/6 x SJL mice. Founder mice were analyzed by Southern blotting and PCR (see Fernandez et al., 2001), using specific primers and backcrossed to C57BL/6 mice to generate the lines pGFAPtTA (GFAP) and TetOCnA (CnA). The astrocyte inducible calcineurin (AIC) mice were generated by crossing the GFAP7 (F3) with the CnA5 (F3) mice.
Phenotypic informationCalcineurin inducible inflammatory phenotype in astrocytes.
Breeding historyThe AIC strain was backcrossed to C57BL/6 for 10 generations. It is currently bred as homozygous male x homozygous female.
References
  • Calcineurin in reactive astrocytes plays a key role in the interplay between proinflammatory and anti-inflammatory signals.;Fernandez Ana M, Fernandez Silvia, Carrero Paloma, Garcia-Garcia Miguel, Torres-Aleman Ignacio, ;2007;The Journal of neuroscience : the official journal of the Society for Neuroscience;27;8745-56; 17699657
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivinghomozygous C57BL/6, homozygous C57BL/6
Stage of embryos2-cell

Literature references

  • Calcineurin in reactive astrocytes plays a key role in the interplay between proinflammatory and anti-inflammatory signals.;Fernandez Ana M, Fernandez Silvia, Carrero Paloma, Garcia-Garcia Miguel, Torres-Aleman Ignacio, ;2007;The Journal of neuroscience : the official journal of the Society for Neuroscience;27;8745-56; 17699657

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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