B6.129-Il2rbtm1Mak/Cnbc

Status

Available to order

EMMA IDEM:06371
International strain nameB6.129-Il2rbtm1Mak/Cnbc
Alternative nameIL - 2 Rb
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolIl2rbtm1Mak,
Gene/Transgene symbolIl2rb

Information from provider

ProviderTak W Mak
Provider affiliationMak Lab, Campbell Family Breast Cancer reseach Institute
Genetic informationMice defective in IL-2Rb expression were generated by insertion of neomycin resistance cassette into Il2rb gene at exon 6 which encodes a region of the extracellular domain proximal to the transmembrane region.
Phenotypic informationHomozygous mice are smaller, have abnormal hair, slow movement and poorly developed external genitals. Death occurs at 12 weeks of age. T cells are spontaneously activated causing exhaustive differentiation of B cells into plasma cells and high serum concentrations of IgG1 and IgE, as well as autoantibodies that cause hemolytic anemia.
Breeding history10 backcrosses to C57BL/6.
References
  • Deregulated T cell activation and autoimmunity in mice lacking interleukin-2 receptor beta.;Suzuki H, Kündig T M, Furlonger C, Wakeham A, Timms E, Matsuyama T, Schmits R, Simard J J, Ohashi P S, Griesser H, ;1995;Science (New York, N.Y.);268;1472-6; 7770771
  • Abnormal development of intestinal intraepithelial lymphocytes and peripheral natural killer cells in mice lacking the IL-2 receptor beta chain.;Suzuki H, Duncan G S, Takimoto H, Mak T W, ;1997;The Journal of experimental medicine;185;499-505; 9053450
  • Normal regulatory alpha/beta T cells effectively eliminate abnormally activated T cells lacking the interleukin 2 receptor beta in vivo.;Suzuki H, Zhou Y W, Kato M, Mak T W, Nakashima I, ;1999;The Journal of experimental medicine;190;1561-72; 10587347
Homozygous fertileno
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedyes

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6J, wild-type C57BL/6J
Stage of embryos2-cell

Disease and phenotype information

MGI phenotypes (allele matching)
  • enlarged spleen / MGI
  • enlarged lymph nodes / MGI
  • abnormal T cell activation / MGI
  • abnormal spleen periarteriolar lymphoid sheath morphology / MGI
  • abnormal lymphocyte morphology / MGI
  • increased anti-double stranded DNA antibody level / MGI
  • increased susceptibility to autoimmune hemolytic anemia / MGI
  • decreased regulatory T cell number / MGI
  • decreased T cell proliferation / MGI
  • abnormal response to transplant / MGI
  • liver inflammation / MGI
  • premature death / MGI
  • abnormal immune system organ morphology / MGI
  • abnormal T cell clonal deletion / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • abnormal marginal zone B cell morphology / MGI
  • increased neutrophil cell number / MGI
  • increased granulocyte number / MGI
  • abnormal sex gland morphology / MGI
  • weight loss / MGI
  • abnormal locomotor behavior / MGI
  • thymus hypoplasia / MGI
  • increased activated T cell number / MGI
  • abnormal hair texture / MGI
  • increased IgE level / MGI
  • increased erythroid progenitor cell number / MGI
  • increased anti-nuclear antigen antibody level / MGI
  • decreased B cell number / MGI
  • decreased double-positive T cell number / MGI
  • abnormal common myeloid progenitor cell morphology / MGI
  • decreased memory T cell number / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • increased plasma cell number / MGI
  • abnormal B cell activation / MGI
  • abnormal mononuclear cell morphology / MGI
  • increased IgG1 level / MGI

Literature references

  • Deregulated T cell activation and autoimmunity in mice lacking interleukin-2 receptor beta.;Suzuki H, Kündig T M, Furlonger C, Wakeham A, Timms E, Matsuyama T, Schmits R, Simard J J, Ohashi P S, Griesser H, ;1995;Science (New York, N.Y.);268;1472-6; 7770771
  • Abnormal development of intestinal intraepithelial lymphocytes and peripheral natural killer cells in mice lacking the IL-2 receptor beta chain.;Suzuki H, Duncan G S, Takimoto H, Mak T W, ;1997;The Journal of experimental medicine;185;499-505; 9053450
  • Normal regulatory alpha/beta T cells effectively eliminate abnormally activated T cells lacking the interleukin 2 receptor beta in vivo.;Suzuki H, Zhou Y W, Kato M, Mak T W, Nakashima I, ;1999;The Journal of experimental medicine;190;1561-72; 10587347

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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