B6;129-Engtm1Hma/Cnrm

Status

Available to order

EMMA IDEM:00066
International strain nameB6;129-Engtm1Hma/Cnrm
Alternative nameEng-KO, CD105-KO
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolEngtm1Hma,
Gene/Transgene symbolEng

Information from provider

ProviderHelen Arthur
Provider affiliationMedical School, University of Newcastle upon Tyne
Genetic informationThe construct contained a neo-targeted deletion of the central portion of the endoglin (Eng, formerly Cd105) gene and also carried a neo gene. The targeted line was produced by homologous recombination in ES cells.
Phenotypic informationEmbryonic lethal at 10.5 dpc. At 9.5 dpc, Eng -/- embryos display defective angiogenesis of the yolk sac. Many Eng -/- embryos also show signs of abnormal cardiac development, including failed organisation of the endocardial cushions and pericardial effusion. Heterozygous animals show defects in vascular smooth muscle organisation. Fragile vessels near epidermal surfaces are vulnerable to trauma and may haemorrhage.
References
  • Endoglin, an ancillary TGFbeta receptor, is required for extraembryonic angiogenesis and plays a key role in heart development.;Arthur H M, Ure J, Smith A J, Renforth G, Wilson D I, Torsney E, Charlton R, Parums D V, Jowett T, Marchuk D A, Burn J, Diamond A G, ;2000;Developmental biology;217;42-53; 10625534

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • abnormal blood vessel morphology / MGI
  • abnormal vascular smooth muscle morphology / MGI
  • abnormal heart development / MGI
  • abnormal heart looping / MGI
  • anemia / MGI
  • decreased embryo size / MGI
  • thin myocardium / MGI
  • abnormal vitelline vasculature morphology / MGI
  • abnormal embryonic hematopoiesis / MGI
  • abnormal capillary morphology / MGI
  • embryonic growth retardation / MGI
  • abnormal dorsal aorta morphology / MGI
  • abnormal heart ventricle morphology / MGI
  • pericardial effusion / MGI
  • abnormal cardiac outflow tract development / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • mortality/aging / MGI

Literature references

  • Endoglin, an ancillary TGFbeta receptor, is required for extraembryonic angiogenesis and plays a key role in heart development.;Arthur H M, Ure J, Smith A J, Renforth G, Wilson D I, Torsney E, Charlton R, Parums D V, Jowett T, Marchuk D A, Burn J, Diamond A G, ;2000;Developmental biology;217;42-53; 10625534

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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