- increased granulocyte number / MGI
- abnormal spleen morphology / MGI
- spleen hypoplasia / MGI
- decreased body size / MGI
- arrested B cell differentiation / MGI
- decreased IgM level / MGI
- thymus hypoplasia / MGI
- arrested T cell differentiation / MGI
- increased susceptibility to infection / MGI
- abnormal effector T cell morphology / MGI
- increased natural killer cell mediated cytotoxicity / MGI
- decreased double-positive T cell number / MGI
- increased macrophage cell number / MGI
- decreased CD4-positive, alpha beta T cell number / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- lymph node hypoplasia / MGI
- absent mature B cells / MGI
- abnormal T cell receptor V(D)J recombination / MGI
- abnormal immunoglobulin V(D)J recombination / MGI
- decreased thymocyte number / MGI
- lung inflammation / MGI
- increased susceptibility to bacterial infection / MGI
- abnormal macrophage physiology / MGI
- colitis / MGI
- abnormal T-helper 1 physiology / MGI
- abnormal response to transplant / MGI
- abnormal interferon secretion / MGI
- abnormal chemokine secretion / MGI
- decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
- abnormal intestinal mucosa morphology / MGI
- abnormal B cell morphology / MGI
- decreased susceptibility to parasitic infection / MGI
- decreased double-negative T cell number / MGI
- increased double-negative T cell number / MGI
- decreased susceptibility to type IV hypersensitivity reaction / MGI
- abnormal stomach mucosa morphology / MGI
- abnormal intestinal epithelium morphology / MGI
- abnormal B cell number / MGI
- decreased pre-B cell number / MGI
- decreased mature B cell number / MGI
- increased immature B cell number / MGI
- decreased immature B cell number / MGI
- abnormal gamma-delta T cell differentiation / MGI
- autoimmune response / MGI
STOCK Rag2tm1Fwa Ifnar1tm Tg(TcrLCMV)327Sdz/Orl
Status | Available to order |
EMMA ID | EM:07003 |
International strain name | STOCK Rag2tm1Fwa Ifnar1tm Tg(TcrLCMV)327Sdz/Orl |
Alternative name | P14TCR-TG/Rag2-/-/InfaR-/- mice |
Strain type | Transgenic Strains |
Allele/Transgene symbol | Tg(TcrLCMV)327Sdz, |
Gene/Transgene symbol | Tg(TcrLCMV)327Sdz |
Information from provider
Provider | Benedita Rocha |
Provider affiliation | INSERM U 1020, INSERM |
Additional owner | I am exclusive owner of this crossing, but not of the individual strains used for the crossing that are generaly available without restrictions. |
Genetic information | This strain was generated by crossing the P14 transgenic mice, which bear a TCR transgene specific for the GP33-peptide of the Leukocyte Choriomeningitis Virus (LCMV), with two deficient mouse strains. The first was deficient in the recombinase Rag2 and the second in the receptor for the interferon alpha (Ifnar1, prev. InfaR). The resulting strain allows the study of the influence of interferon alpha (which is produced in very high amounts during LCMV infection) in the response of CD8 T cells to LCMV. We would cryopreserve this crossing since the fixation in the same mouse strain of 2 targeted mutations and one transgene was very difficult and we believe it will be of major interest to the immunology community. |
Phenotypic information | Mice will have a single population of lymphocytes, those expressing the TCR transgene. |
Breeding history | Currently bred homozygous for the Rag2 mutation and the TCR transgene, heterozygous for the Ifnar1 (InfarR) mutation. |
References | None available |
Homozygous fertile | no |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | yes |
Information from EMMA
Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Animals used for archiving | homozygous C57BL/6J, wild-type C57BL/6J |
Breeding at archiving centre | Males knock-out for Rag2 and Ifnar1 (InfaR), homozygous transgenic for Tg(TcrLCMV)327Sdz (P14TCR). Females heterozygous or wild-type for each mutation. |
Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Severe combined immunodeficiency due to complete RAG1/2 deficiency / Orphanet_331206
- Omenn syndrome / Orphanet_39041
- Combined immunodeficiency with granulomatosis / Orphanet_157949
MGI phenotypes (allele matching)
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