- no phenotypic analysis / MGI
STOCK Gt(ROSA)26Sortm1(CAG-mCherry/Rpl10a)Sten/Kctt
Status | Available to order |
EMMA ID | EM:07451 |
International strain name | STOCK Gt(ROSA)26Sortm1(CAG-mCherry/Rpl10a)Sten/Kctt |
Alternative name | Gt(ROSA)26Sor-mCherry-Rpl10a |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Gt(ROSA)26Sortm1(CAG-mCherry/Rpl10a)Sten, |
Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
Provider | Jan Stenman |
Provider affiliation | Ludwig Institute for Cancer Research Ltd |
Genetic information | To generate the Gt(ROSA)26Sor-mCherry-Rpl10a allele, we PCR amplified mCherry from mCherry-pRSET-B (provided by Roger Tsien), and Rpl10a from CS-EGFP-L10A (provided by Nathaniel Heintz). mCherry was digested with NheI/XhoI, Rpl10a with XhoI/NotI, and subcloned into the NheI/NotI sites of pBSApBpACAGftILn. Finally, the conditional expression construct was released with PacI and AscI, and subcloned into the PacI and AscI sites of pRosa26PAS. The construct was linearized and electroporated into F1 ES cells. Colonies were screened by PCR and positive colonies expanded and confirmed by PCR. One targeted clone was injected into host C57BL/6J (The Jackson Laboratory) blastocysts by the Karolinska Center for Transgene Technologies, Karolinska Institutet. |
Phenotypic information | None |
Breeding history | mixed background |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | Karolinska Institutet, Stockholm, Sweden |
Animals used for archiving | homozygous 0, wild-type 0 |
Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
Literature references
- Evaluation of TRAP-sequencing technology with a versatile conditional mouse model.;Hupe Mike, Li Minerva Xueting, Gertow Gillner Karin, Adams Ralf H, Stenman Jan M, ;2014;Nucleic acids research;42;e14; 24165879
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