A.B6-Prf1tm1Sdz/Biat

Status

Available to order

EMMA IDEM:07765
International strain nameA.B6-Prf1tm1Sdz/Biat
Alternative nameAJ.PKO-/- or AJ.PKO-/+
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolPrf1tm1Sdz,
Gene/Transgene symbolPrf1

Information from provider

ProviderSteffen Frese
Provider affiliationUniversity of Bern
Genetic informationA/J mice which are susceptible for chemically (tobacco carcinogen NNK)-induced lung cancer were backcrossed for more than 10 generations with C57BL/6 perforin knock-out mice provided by Prof. Hengartner from University of Zurich.
Phenotypic informationOur data showed that these mice are more susceptible for chemically induced lung cancer (Frese-Schaper M. et al., Cancer Immunol Immunother, 12014).
Breeding historyThe C57BL/6 mouse strain deficient in perforin was received from Hengartner's laboratory in 2009 and backcrossed for more than 10 generations to A/J background. A/J mice can be used as a model for chemically induced lung tumors.
References
  • Decreased tumor surveillance in perforin-deficient mice.;van den Broek M E, Kägi D, Ossendorp F, Toes R, Vamvakas S, Lutz W K, Melief C J, Zinkernagel R M, Hengartner H, ;1996;The Journal of experimental medicine;184;1781-90; 8920866
  • Influence of natural killer cells and perforin‑mediated cytolysis on the development of chemically induced lung cancer in A/J mice.;Frese-Schaper Manuela, Keil Andreas, Yagita Hideo, Steiner Selina Katja, Falk Werner, Schmid Ralph Alexander, Frese Steffen, ;2014;Cancer immunology, immunotherapy : CII;63;571-80; 24658838
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisednot known

Information from EMMA

Archiving centreUniversity of Veterinary Medicine, Vienna, Austria
Animals used for archivinghomozygous A/J

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • insulitis / MGI
  • decreased susceptibility to autoimmune diabetes / MGI
  • periinsulitis / MGI
  • increased incidence of induced tumors / MGI
  • impaired natural killer cell mediated cytotoxicity / MGI
  • decreased leukocyte cell number / MGI
  • decreased neutrophil cell number / MGI
  • decreased bone marrow cell number / MGI
  • ruffled hair / MGI
  • demyelination / MGI
  • weight loss / MGI
  • hypoactivity / MGI
  • hunched posture / MGI
  • increased circulating triglyceride level / MGI
  • abnormal body temperature homeostasis / MGI
  • abnormal T cell activation / MGI
  • brain inflammation / MGI
  • liver inflammation / MGI
  • abnormal lymph node morphology / MGI
  • abnormal spleen white pulp morphology / MGI
  • increased susceptibility to viral infection / MGI
  • decreased erythrocyte cell number / MGI
  • thrombocytopenia / MGI
  • lymph node inflammation / MGI
  • abnormal lymphocyte physiology / MGI
  • abnormal behavior / MGI
  • abnormal cytotoxic T cell physiology / MGI
  • decreased cytotoxic T cell cytolysis / MGI
  • pancytopenia / MGI
  • lethargy / MGI
  • increased circulating aspartate transaminase level / MGI
  • behavior/neurological phenotype / MGI
  • immune system phenotype / MGI
  • decreased body temperature / MGI
  • CNS inflammation / MGI
  • increased spleen red pulp amount / MGI
  • increased circulating tumor necrosis factor level / MGI
  • decreased interferon-gamma secretion / MGI
  • increased circulating interferon-alpha level / MGI
  • increased circulating interferon-gamma level / MGI
  • increased circulating interleukin-10 level / MGI
  • increased circulating interleukin-6 level / MGI
  • increased circulating interleukin-18 level / MGI
  • increased circulating interleukin-1 beta level / MGI
  • decreased NK cell degranulation / MGI
  • abnormal brain meninges morphology / MGI
  • increased susceptibility to viral infection induced morbidity/mortality / MGI
  • abnormal circulating cytokine level / MGI
  • abnormal circulating fibrinogen level / MGI
  • abnormal macrophage activation involved in immune response / MGI
  • increased circulating lactate dehydrogenase level / MGI
  • abnormal inflammatory response / MGI
  • increased B cell derived lymphoma incidence / MGI
  • premature death / MGI
  • increased susceptibility to infection / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal leukocyte physiology / MGI
  • abnormal female reproductive system physiology / MGI
  • increased susceptibility to parasitic infection / MGI
  • increased interleukin-10 secretion / MGI
  • increased sensitivity to induced morbidity/mortality / MGI
  • decreased tumor latency / MGI
  • abnormal NK cell physiology / MGI
  • increased lymphoma incidence / MGI
  • abnormal circulating glucose level / MGI
  • decreased pancreatic beta cell number / MGI
  • increased physiological sensitivity to xenobiotic / MGI
  • increased T cell derived lymphoma incidence / MGI
  • increased lung adenocarcinoma incidence / MGI
  • increased sarcoma incidence / MGI
  • abnormal tumor morphology / MGI
  • increased tumor latency / MGI

Literature references

  • Decreased tumor surveillance in perforin-deficient mice.;van den Broek M E, Kägi D, Ossendorp F, Toes R, Vamvakas S, Lutz W K, Melief C J, Zinkernagel R M, Hengartner H, ;1996;The Journal of experimental medicine;184;1781-90; 8920866
  • Influence of natural killer cells and perforin‑mediated cytolysis on the development of chemically induced lung cancer in A/J mice.;Frese-Schaper Manuela, Keil Andreas, Yagita Hideo, Steiner Selina Katja, Falk Werner, Schmid Ralph Alexander, Frese Steffen, ;2014;Cancer immunology, immunotherapy : CII;63;571-80; 24658838

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

Other EMMA strains

Not found what you were looking for? Search here for other strains available from EMMA.


Search
INFRAFRONTIER® and European Mouse Mutant Archive - EMMA® are registered trademarks at the European Union Intellectual Property Office (EUIPO).