B6(Cg)-Map3k14aly/Ieg

Status

Available to order

EMMA IDEM:07800
International strain nameB6(Cg)-Map3k14aly/Ieg
Alternative nameC57BL/6J-Map3k14 N12
Strain typeSpontaneous
Allele/Transgene symbolMap3k14aly,
Gene/Transgene symbolMap3k14

Information from provider

ProviderJoerg Kirberg
Provider affiliationDiv. of Immunology (3/3), Paul-Ehrlich-Institut
Genetic informationMice carry the known mutation of Map3k14 (mitogen-activated protein kinase kinase kinase 14, aka NIK, NF-kB inducing kinase) termed aly (alymphoplasia). That mutation arose spontaneously on a mixed background (AEJ/GnRk * C57BL/6J; The Jackson Lab ref. J:16865, PubMed ref. PMID 8299692) and was later identified as G-to-A transition point mutation resulting in a replacement of a conserved glycine for an arginine at position 855 of the encoded protein (The Jackson Lab ref. J:54529; PubMed ref. PMID 10319865). The mutant allele was reported to be bred to C57BL/6. However, variations in the proportions of marginal B cells in the spleen, reported to be absent in aly mice, were observed here. For this reason, the mutant allele was backcrossed to C57BL/6 for 12 generations (exchanging the sex chromosomes along).
Phenotypic informationHomozygous:
Alymphoplasia, see The Jackson Lab ref. J:16865, PubMed ref. PMID 8299692. Underlying mutation: see The Jackson Lab ref. J:54529, PubMed ref. PMID 10319865.

Heterozygous:
nothing obvious
Breeding historyThe mutant allele was backcrossed to C57BL/6 for 12 generations (exchanging the sex chromosomes along). Following this, the line was expanded by intercrossing to obtain aly/aly homozygotes. Since aly/aly females are supposedly unable to lactate, the line is maintained as aly heterozygous females mated with aly/aly homozygous males.
References
  • A new mutation, aly, that induces a generalized lack of lymph nodes accompanied by immunodeficiency in mice.;Miyawaki S, Nakamura Y, Suzuka H, Koba M, Yasumizu R, Ikehara S, Shibata Y, ;1994;European journal of immunology;24;429-34; 8299692
  • Alymphoplasia is caused by a point mutation in the mouse gene encoding Nf-kappa b-inducing kinase.;Shinkura R, Kitada K, Matsuda F, Tashiro K, Ikuta K, Suzuki M, Kogishi K, Serikawa T, Honjo T, ;1999;Nature genetics;22;74-7; 10319865
Homozygous fertilemales only
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedyes

Information from EMMA

Archiving centreHelmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archivingheterozygous C57BL/6J, wild-type C57BL/6J
Stage of embryos2-cell

Disease and phenotype information

MGI allele-associated human disease models

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • premature death / MGI
  • increased susceptibility to infection / MGI
  • absent lymph nodes / MGI
  • decreased mast cell number / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal salivary gland morphology / MGI
  • abnormal mammary gland development / MGI
  • abnormal immune system morphology / MGI
  • small thymus / MGI
  • abnormal immune system physiology / MGI
  • abnormal T cell activation / MGI
  • increased inflammatory response / MGI
  • kidney inflammation / MGI
  • liver inflammation / MGI
  • lung inflammation / MGI
  • pancreas inflammation / MGI
  • abnormal hepatobiliary system morphology / MGI
  • abnormal thymus medulla morphology / MGI
  • abnormal dendritic cell physiology / MGI
  • abnormal dendritic cell antigen presentation / MGI
  • decreased IgE level / MGI
  • absent Peyer's patches / MGI
  • abnormal bile duct morphology / MGI
  • dilated bile duct / MGI
  • bile duct inflammation / MGI
  • bile duct proliferation / MGI
  • abnormal intestinal goblet cell morphology / MGI
  • bile duct hyperplasia / MGI
  • decreased regulatory T cell number / MGI
  • increased eosinophil cell number / MGI
  • increased susceptibility to parasitic infection / MGI
  • abnormal NK T cell morphology / MGI
  • decreased NK T cell number / MGI
  • decreased dendritic cell number / MGI
  • abnormal CD4-positive, CD25-positive, alpha-beta regulatory T cell morphology / MGI
  • decreased circulating interferon-gamma level / MGI
  • decreased circulating interleukin-4 level / MGI
  • lacrimal gland inflammation / MGI
  • abnormal mammary fat pad morphology / MGI
  • decreased mammary fat pad weight / MGI
  • abnormal thymus corticomedullary boundary morphology / MGI
  • thymus cysts / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal lymphocyte morphology / MGI
  • increased length of allograft survival / MGI
  • decreased B cell number / MGI
  • absent follicular dendritic cells / MGI
  • absent spleen marginal zone / MGI
  • abnormal spleen B cell follicle morphology / MGI
  • abnormal spleen primary B follicle morphology / MGI
  • mortality/aging / MGI
  • absent thymus corticomedullary boundary / MGI

Literature references

  • A new mutation, aly, that induces a generalized lack of lymph nodes accompanied by immunodeficiency in mice.;Miyawaki S, Nakamura Y, Suzuka H, Koba M, Yasumizu R, Ikehara S, Shibata Y, ;1994;European journal of immunology;24;429-34; 8299692
  • Alymphoplasia is caused by a point mutation in the mouse gene encoding Nf-kappa b-inducing kinase.;Shinkura R, Kitada K, Matsuda F, Tashiro K, Ikuta K, Suzuki M, Kogishi K, Serikawa T, Honjo T, ;1999;Nature genetics;22;74-7; 10319865

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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